CRISPR screens in plants: approaches, guidelines, and future prospects

C Gaillochet, W Develtere, TB Jacobs - The Plant Cell, 2021 - academic.oup.com
Clustered regularly interspaced short palindromic repeat (CRISPR)-associated systems
have revolutionized genome engineering by facilitating a wide range of targeted DNA …

Brachypodium distachyon and Setaria viridis: Model Genetic Systems for the Grasses

TP Brutnell, JL Bennetzen… - Annual review of plant …, 2015 - annualreviews.org
The family of grasses encompasses the world's most important food, feed, and bioenergy
crops, yet we are only now beginning to develop the genetic resources to explore the …

An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii

X Li, R Zhang, W Patena, SS Gang, SR Blum… - The Plant …, 2016 - academic.oup.com
The green alga Chlamydomonas reinhardtii is a leading unicellular model for dissecting
biological processes in photosynthetic eukaryotes. However, its usefulness has been limited …

The maize W22 genome provides a foundation for functional genomics and transposon biology

NM Springer, SN Anderson, CM Andorf, KR Ahern… - Nature …, 2018 - nature.com
The maize W22 inbred has served as a platform for maize genetics since the mid twentieth
century. To streamline maize genome analyses, we have sequenced and de novo …

Evolutionarily informed machine learning enhances the power of predictive gene-to-phenotype relationships

CY Cheng, Y Li, K Varala, J Bubert, J Huang… - Nature …, 2021 - nature.com
Inferring phenotypic outcomes from genomic features is both a promise and challenge for
systems biology. Using gene expression data to predict phenotypic outcomes, and …

Dicer-like 5 deficiency confers temperature-sensitive male sterility in maize

C Teng, H Zhang, R Hammond, K Huang… - Nature …, 2020 - nature.com
Small RNAs play important roles during plant development by regulating transcript levels of
target mRNAs, maintaining genome integrity, and reinforcing DNA methylation. Dicer-like 5 …

A kinesin-14 motor activates neocentromeres to promote meiotic drive in maize

RK Dawe, EG Lowry, JI Gent, MC Stitzer… - Cell, 2018 - cell.com
Summary Maize abnormal chromosome 10 (Ab10) encodes a classic example of true
meiotic drive that converts heterochromatic regions called knobs into motile neocentromeres …

Genome‐wide LORE1 retrotransposon mutagenesis and high‐throughput insertion detection in Lotus japonicus

DF Urbański, A Małolepszy, J Stougaard… - The Plant …, 2012 - Wiley Online Library
Use of insertion mutants facilitates functional analysis of genes, but it has been difficult to
identify a suitable mutagen and to establish large populations for reverse genetics in most …

An RNA recognition motif-containing protein is required for plastid RNA editing in Arabidopsis and maize

T Sun, A Germain, L Giloteaux… - Proceedings of the …, 2013 - National Acad Sciences
Plant RNA editing modifies cytidines (C) to uridines (U) at specific sites in the transcripts of
both mitochondria and plastids. Specific targeting of particular Cs is achieved by …

Regulation of seed vigor by manipulation of raffinose family oligosaccharides in maize and Arabidopsis thaliana

T Li, Y Zhang, D Wang, Y Liu, LMA Dirk, J Goodman… - Molecular Plant, 2017 - cell.com
Raffinose family oligosaccharides (RFOs) accumulate in seeds during maturation
desiccation in many plant species. However, it remains unclear whether RFOs have a role in …