PCR in laboratory diagnosis of human Borrelia burgdorferi infections

BL Schmidt - Clinical microbiology reviews, 1997 - Am Soc Microbiol
The laboratory diagnosis of Lyme borreliosis, the most prevalent vector-borne disease in the
United States and endemic in parts of Europe and Asia, is currently based on serology with …

Analysis of the 16S rRNA gene of microorganism WSU 86-1044 from an aborted bovine foetus reveals that it is a member of the order Chlamydiales: proposal of Waddliaceae …

FR Rurangirwa, PM Dilbeck… - … of Systematic and …, 1999 - microbiologyresearch.org
The structural gene encoding the 16S rRNA of the new obligate intracellular organism
presently designated WSU 86-1044T was sequenced and analysed to establish its …

Relation between antioxidant enzyme gene expression and antioxidative defense status of insulin-producing cells

M Tiedge, S Lortz, J Drinkgern, S Lenzen - Diabetes, 1997 - Am Diabetes Assoc
Antioxidant enzyme expression was determined in rat pancreatic islets and RINm5F insulin-
producing cells on the level of mRNA, protein, and enzyme activity in comparison with 11 …

PCR Fusion-Based Approach to Create Reporter Gene Constructs for Expression Analysis in Transgenic C. elegans

O Hobert - Biotechniques, 2002 - Taylor & Francis
In the nematode Caenorhabditis elegans, gene expression patterns are most often
determined by generating a fusion of the promoter of the gene under study to a reporter …

PCR-mediated recombination and mutagenesis: SOEing together tailor-made genes

RM Horton - Molecular biotechnology, 1995 - Springer
Abstract Gene Splicing by Overlap Extension (gene SOEing) is a sequence-independent
method for site-directed mutagenesis and/or recombination of DNA molecules. It is based on …

A technique whose time has come

NJ Walker - Science, 2002 - science.org
A Technique Whose Time Has Come | Science news careers commentary Journals Science
Science brought to you byGoogle Indexer Log in science science advances science immunology …

Paracrine expression of a native soluble vascular endothelial growth factor receptor inhibits tumor growth, metastasis, and mortality rate

CK Goldman, RL Kendall, G Cabrera… - Proceedings of the …, 1998 - National Acad Sciences
Vascular endothelial growth factor (VEGF) is a potent and selective vascular endothelial cell
mitogen and angiogenic factor. VEGF expression is elevated in a wide variety of solid …

Complementary action of antioxidant enzymes in the protection of bioengineered insulin-producing RINm5F cells against the toxicity of reactive oxygen species.

M Tiedge, S Lortz, R Munday, S Lenzen - Diabetes, 1998 - Am Diabetes Assoc
To determine the importance of different antioxidative enzymes for the defense status of
insulin-producing cells, the effects of stable overexpression of glutathione peroxidase (Gpx) …

[图书][B] Molecular detection of human bacterial pathogens

D Liu - 2011 - books.google.com
As more original molecular protocols and subsequent modifications are described in the
literature, it has become difficult for those not directly involved in the development of these …

Real‐time and quantitative PCR: applications to mechanism‐based toxicology

NJ Walker - Journal of biochemical and molecular toxicology, 2001 - Wiley Online Library
There is increasing awareness that quantitative analysis of changes in molecular targets
plays a key role in addressing scientific questions in molecular toxicology, molecular …