An ATP-powered DNA translocation machine encapsidates the viral genome in the large
dsDNA bacteriophages. The essential components include the empty shell, prohead, and …

To fully understand biological processes from the metabolism of a bacterium to the operation
of a human brain, it is necessary to know the three-dimensional (3D) spatial arrangement …

Cryoelectron microscopy of biological molecules is among the hottest growth areas in
biophysics and structural biology at present, and Frank is arguably the most distinguished …

The identification of proximate amino acids by chemical cross-linking and mass
spectrometry (XL-MS) facilitates the structural analysis of homogeneous protein complexes …

Nanobodies are single-domain antibodies of camelid origin. We generated nanobodies
against the vertebrate nuclear pore complex (NPC) and used them in STORM imaging to …

Compartmentalization is an important organizational feature of life. It occurs at varying levels
of complexity ranging from eukaryotic organelles and the bacterial microcompartments, to …

Septins are GTP-binding proteins that assemble into homo-and hetero-oligomers and
filaments. Although they have key roles in various cellular processes, little is known …

Ds-DNA viruses package their DNA into a preformed protein shell (procapsid) during
maturation. Bacteriophage φ29 requires an RNA (pRNA) to package its genomic DNA into …

Motors generating mechanical force, powered by the hydrolysis of ATP, translocate double-
stranded DNA into preformed capsids (proheads) of bacterial viruses, and certain animal …

The spliceosomal B complex is the substrate that undergoes catalytic activation leading to
catalysis of pre-mRNA splicing. Previous characterization of this complex was performed in …