A guide to light-sheet fluorescence microscopy for multiscale imaging
RM Power, J Huisken - Nature methods, 2017 - nature.com
The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as
developmental and cell biology, anatomical science, biophysics and neuroscience …
developmental and cell biology, anatomical science, biophysics and neuroscience …
Phototoxicity in live fluorescence microscopy, and how to avoid it
Phototoxicity frequently occurs during live fluorescence microscopy, and its consequences
are often underestimated. Damage to cellular macromolecules upon excitation light …
are often underestimated. Damage to cellular macromolecules upon excitation light …
Assessing phototoxicity in live fluorescence imaging
Are the answers to biological questions obtained via live fluorescence microscopy
substantially affected by phototoxicity? Although a single set of standards for assessing …
substantially affected by phototoxicity? Although a single set of standards for assessing …
A highly luminescent nitrogen-doped nanographene as an acid-and metal-sensitive fluorophore for optical imaging
Dibenzo [hi, st] ovalene (DBOV) has excellent photophysical properties, including strong
fluorescence and high ambient stability. Moreover, the optical blinking properties of DBOV …
fluorescence and high ambient stability. Moreover, the optical blinking properties of DBOV …
Real-time 3D single molecule tracking
To date, single molecule studies have been reliant on tethering or confinement to achieve
long duration and high temporal resolution measurements. Here, we present a 3D single …
long duration and high temporal resolution measurements. Here, we present a 3D single …
High-resolution low-power hyperspectral line-scan imaging of fast cellular dynamics using azo-enhanced Raman scattering probes
Small-molecule Raman probes for cellular imaging have attracted great attention owing to
their sharp peaks that are sensitive to environmental changes. The small cross section of …
their sharp peaks that are sensitive to environmental changes. The small cross section of …
In vivo characterisation of fluorescent proteins in budding yeast
Fluorescent proteins (FPs) are widely used in many organisms, but are commonly
characterised in vitro. However, the in vitro properties may poorly reflect in vivo performance …
characterised in vitro. However, the in vitro properties may poorly reflect in vivo performance …
Hierarchical vessel network-supported tumor model-on-a-chip constructed by induced spontaneous anastomosis
The vascular system in living tissues is a highly organized system that consists of vessels
with various diameters for nutrient delivery and waste transport. In recent years, many vessel …
with various diameters for nutrient delivery and waste transport. In recent years, many vessel …
Recent advances in experimental design and data analysis to characterize prokaryotic motility
MM Dubay, J Acres, M Riekeles, JL Nadeau - Journal of Microbiological …, 2023 - Elsevier
Bacterial motility plays a key role in important cell processes such as chemotaxis and biofilm
formation, but is challenging to quantify due to the small size of the individual …
formation, but is challenging to quantify due to the small size of the individual …
Between life and death: strategies to reduce phototoxicity in super-resolution microscopy
KL Tosheva, Y Yuan, PM Pereira… - Journal of Physics D …, 2020 - iopscience.iop.org
Super-resolution microscopy (SRM) enables non-invasive, molecule-specific imaging of the
internal structure and dynamics of cells with sub-diffraction limit spatial resolution. One of its …
internal structure and dynamics of cells with sub-diffraction limit spatial resolution. One of its …