Twisted intramolecular charge transfer (TICT) and twists beyond TICT: from mechanisms to rational designs of bright and sensitive fluorophores
The twisted intramolecular charge transfer (TICT) mechanism has guided the development
of numerous bright and sensitive fluorophores. This review briefly overviews the history of …
of numerous bright and sensitive fluorophores. This review briefly overviews the history of …
Spontaneously Blinking Rhodamine Dyes for Single‐Molecule Localization Microscopy
Single‐molecule localization microscopy (SMLM) has found extensive applications in
various fields of biology and chemistry. As a vital component of SMLM, fluorophores play an …
various fields of biology and chemistry. As a vital component of SMLM, fluorophores play an …
Field-dependent deep learning enables high-throughput whole-cell 3D super-resolution imaging
Single-molecule localization microscopy in a typical wide-field setup has been widely used
for investigating subcellular structures with super resolution; however, field-dependent …
for investigating subcellular structures with super resolution; however, field-dependent …
Monitoring amyloid aggregation via a twisted intramolecular charge transfer (TICT)-based fluorescent sensor array
Imaging amyloid-beta (Aβ) aggregation is critical for understanding the pathology and aiding
the pre-symptomatic intervention of Alzheimer's disease (AD). Amyloid aggregation consists …
the pre-symptomatic intervention of Alzheimer's disease (AD). Amyloid aggregation consists …
Long-term super-resolution inner mitochondrial membrane imaging with a lipid probe
The inner mitochondrial membrane (IMM) generates power to drive cell function, and its
dynamics control mitochondrial health and cellular homeostasis. Here, we describe the cell …
dynamics control mitochondrial health and cellular homeostasis. Here, we describe the cell …
THQ–Xanthene: An Emerging Strategy to Create Next‐Generation NIR‐I/II Fluorophores
Near‐infrared fluorescence imaging is vital for exploring the biological world. The short
emissions (< 650 nm) and small Stokes shifts (< 30 nm) of current xanthene dyes obstruct …
emissions (< 650 nm) and small Stokes shifts (< 30 nm) of current xanthene dyes obstruct …
Photochemical mechanisms of fluorophores employed in single‐molecule localization microscopy
Decoding cellular processes requires visualization of the spatial distribution and dynamic
interactions of biomolecules. It is therefore not surprising that innovations in imaging …
interactions of biomolecules. It is therefore not surprising that innovations in imaging …
Overcoming Spectral Dependence: A General Strategy for Developing Far‐Red and Near‐Infrared Ultra‐Fluorogenic Tetrazine Bioorthogonal Probes
Bioorthogonal fluorogenic dyes are indispensable tools in wash‐free bioimaging of specific
biological targets. However, the fluorogenicity of existing tetrazine‐based bioorthogonal …
biological targets. However, the fluorogenicity of existing tetrazine‐based bioorthogonal …
Recruiting rate determines the blinking propensity of rhodamine fluorophores for super-resolution imaging
Y Zheng, Z Ye, X Zhang, Y Xiao - Journal of the American …, 2023 - ACS Publications
Live-cell single-molecule localization microscopy has advanced with the development of self-
blinking rhodamines. A p K cycling of< 6 is recognized as the criterion for self-blinking, yet a …
blinking rhodamines. A p K cycling of< 6 is recognized as the criterion for self-blinking, yet a …
An Acid‐Regulated Self‐Blinking Fluorescent Probe for Resolving Whole‐Cell Lysosomes with Long‐Term Nanoscopy
Long‐term super‐resolution imaging appears to be increasingly important for unraveling
organelle dynamics at the nanoscale, but is challenging due to the need for highly …
organelle dynamics at the nanoscale, but is challenging due to the need for highly …