Proteins adopt different higher-order structures (HOS) to enable their unique biological
functions. Understanding the complexities of protein higher-order structures and dynamics …

Solution-phase hydrogen/deuterium exchange (HDX) coupled to mass spectrometry (MS) is
a widespread tool for structural analysis across academia and the biopharmaceutical …

Electrospray ionization (ESI) generates intact gas-phase ions from analytes in solution for
mass spectrometric investigations. ESI can proceed via different mechanisms. Low …

The folding/misfolding and pharmacological rescue of multidomain ATP-binding cassette
(ABC) C-subfamily transporters, essential for organismal health, remain incompletely …

INTRODUCTION The ubiquitin-proteasome system comprises hundreds of distinct pathways
of degradation, which converge at the step of ubiquitin recognition by the proteasome. Five …

Mass spectrometry-based methods have become increasingly important in structural biology—
in particular for large and dynamic, even heterogeneous assemblies of biomolecules. Native …

Extremotolerant organisms and industry exploit sugars as desiccation protectants, with
trehalose being widely used by both. How sugars, in general, and the hydrolytically stable …

NMR spectroscopy is a key method for studying the structure and dynamics of (large)
multidomain proteins and complexes in solution. It plays a unique role in integrated …

Much of our understanding of protein structure and mechanistic function has been derived
from static high‐resolution structures. As structural biology has continued to evolve it has …

Natural and traditional medicines, being a great source of drugs and drug leads, have
regained wide interests due to the limited success of high-throughput screening of …