Capillary Electrophoresis–Mass Spectrometry for Top-Down Proteomics

Q Wang, Q Wang, G Zhu, L Sun - Annual Review of Analytical …, 2025 - annualreviews.org
Mass spectrometry (MS)-based top-down proteomics (TDP) characterizes proteoforms in
cells, tissues, and biological fluids (eg, human plasma) to better our understanding of protein …

Deciphering nanoparticle protein coronas by capillary isoelectric focusing-mass spectrometry-based top-down proteomics

G Zhu, SA Sadeghi, M Mahmoudi, L Sun - Chemical Communications, 2024 - pubs.rsc.org
The nanoparticle (NP) protein corona significantly influences the outcome of nanomedicine.
We present the first example of top-down proteomics (TDP) measurement of the protein …

[HTML][HTML] Electrophoresis-Correlative Ion Mobility Deepens Single-cell Proteomics in Capillary Electrophoresis Mass Spectrometry

B Shen, F Zhou, P Nemes - Molecular & Cellular Proteomics, 2024 - Elsevier
Detection of trace-sensitive signals is a current challenge in single-cell mass spectrometry
(MS) proteomics. Separation prior to detection improves the fidelity and depth of proteome …

A simple and efficient approach for preparing cationic coating with tunable electroosmotic flow for capillary zone electrophoresis-mass spectrometry-based top-down …

Q Wang, G Gao, F Fang, Q Wang, PK Lundquist… - Analytica Chimica …, 2024 - Elsevier
Background Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has
become a valuable analytical technique in top-down proteomics (TDP). CZE-MS/MS-based …

FREE: Enhanced Feature Representation for Isotopic Envelope Evaluation in Top-Down Mass Spectra Deconvolution

J Zhong, X Song, S Wang - Analytical Chemistry, 2024 - ACS Publications
The aim of deconvolution of top-down mass spectra is to recognize monoisotopic peaks from
the experimental envelopes in raw mass spectra. So accurate assessment of similarity …