Recent investigations in bacteria suggest that membraneless organelles play a crucial role
in the subcellular organization of bacterial cells. However, the biochemical functions and …

Coupled transcription-translation (CTT) is a hallmark of prokaryotic gene expression. CTT
occurs when ribosomes associate with and initiate translation of mRNAs whose transcription …

Once described as mere “bags of enzymes,” bacterial cells are in fact highly organized, with
many macromolecules exhibiting nonuniform localization patterns. Yet the physical and …

Transcription generates local topological and mechanical constraints on the DNA fiber,
leading to the generation of supercoiled chromosome domains in bacteria. However, the …

Intrinsically disordered proteins/regions (IDPs/IDRs) contribute to a diverse array of
molecular functions in eukaryotic systems. There is also growing recognition that …

Numerous examples of microbial phase-separated biomolecular condensates have now
been identified following advances in fluorescence imaging and single molecule microscopy …

RNA degradosomes are multienzyme complexes composed of ribonucleases, RNA
helicases, and metabolic enzymes. RNase E–based degradosomes are widespread in …

Highlights•Biomolecular condensates act as membraneless organelles that assemble via
liquid–liquid phase-separation.•Bacterial transcription and mRNA decay machinery were …

The rise in fluorescence-based imaging techniques over the past 3 decades has improved
the ability of researchers to scrutinize live cell biology at increased spatial and temporal …

Only recently has it been recognized that the transcriptome of bacteria and archaea can be
spatiotemporally regulated. All types of prokaryotic transcripts—rRNAs, tRNAs, mRNAs, and …