Formation of inclusion bodies in bacterial hosts poses a major challenge for large scale
recovery of bioactive proteins. The process of obtaining bioactive protein from inclusion …

A common limitation of recombinant protein production in bacteria is the formation of
insoluble protein aggregates known as inclusion bodies. The propensity of a given protein to …

Bacterial inclusion bodies (IBs) are functional, non-toxic amyloids occurring in recombinant
bacteria showing analogies with secretory granules of the mammalian endocrine system …

Inclusion bodies (IBs) often emerge upon overexpression of recombinant proteins in E. coli.
From IBs, refolding is necessary to generate the native protein that can be further purified to …

Background Many enzymes of industrial interest are not in the market since they are bio-
produced as bacterial inclusion bodies, believed to be biologically inert aggregates of …

Protein aggregation is a major bottleneck during the bacterial production of recombinant
proteins. In general, the induction of gene expression at sub‐optimal growth temperatures …

Since the introduction of recombinant protein expression in the second half of the 1970s, the
growth of the biopharmaceutical field has been rapid and protein therapeutics has come to …

Understanding the structure, functionalities and biology of functional amyloids is an issue of
emerging interest. Inclusion bodies, namely protein clusters formed in recombinant bacteria …

Increasing evidence indicates that protein aggregation in bacteria does not necessarily
imply loss of biological activity. Here, we have investigated the effect of growth-temperature …

Background For a long time IBs were considered to be inactive deposits of accumulated
target proteins. In our previous studies, we discovered IBs containing a high percentage of …