The in vitro production of porcine embryos has presented numerous challenges to
researchers over the past four decades. Some of the problems encountered were specific to …

During the past 6 years, considerable advancement has been achieved in experimental
embryology of pigs. This process was mainly generated by the rapidly increasing need for …

The efficiency of porcine somatic nuclear transfer (born piglets/transferred embryos) is low.
Here, we report a highly efficient protocol using peripubertal gilts as recipients synchronized …

Fertilized mouse zygotes can reprogram somatic cells to a pluripotent state. Human zygotes
might therefore be useful for producing patient-derived pluripotent stem cells. However …

Two major drawbacks hamper the advancement of somatic cell nuclear transfer in domestic
animals. The first is a biological problem that has been studied extensively by many …

The objective of this study was to assess the practical usefulness of morphologically poor
oocytes (MPCOCs) in relation to follicular size and oocyte diameter. Oocytes collected from …

The production of cloned animals is a difficult and complex procedure that requires two
basic steps. First, the cytoplast must be prepared by the enucleation of metaphase II oocytes …

The objective of this study was to examine the effect of cytochalasin B (CB) and/or
demecolcine (Dc) on the remodeling of donor nuclei, nuclear ploidy, and development of …

The present study demonstrated that demecolcine treatment for at least 30 min produces a
membrane protrusion in metaphase II–stage bovine oocytes. The maternal chromosome …

Somatic cell cloning efficiency is determined by many factors. One of the most important
factors is the structure-functional quality of nuclear donor cells. Morphologic criteria that …