Purification of modified therapeutic proteins available on the market: An analysis of chromatography-based strategies

C Sánchez-Trasviña, M Flores-Gatica… - … in Bioengineering and …, 2021 - frontiersin.org
Proteins, which have inherent biorecognition properties, have long been used as
therapeutic agents for the treatment of a wide variety of clinical indications. Protein …

Product development issues for PEGylated proteins

RW Payne, BM Murphy, MC Manning - Pharmaceutical …, 2011 - Taylor & Francis
Covalent attachment of poly (ethylene) glycol (PEG) groups to proteins, a process commonly
called PEGylation, is often used to improve the performance of a protein in vivo. To date, at …

Interaction mechanism of mono‐PEGylated proteins in electrostatic interaction chromatography

M Abe, P Akbarzaderaleh, M Hamachi… - Biotechnology …, 2010 - Wiley Online Library
The retention and binding mechanisms in electrostatic interaction‐based chromatography
(ion‐exchange chromatography) of PEGylated proteins (covalent attachment of polyethylene …

Hydrophobic interaction chromatography for purification of monoPEGylated RNase A

K Mayolo-Deloisa, ME Lienqueo, B Andrews… - … of Chromatography A, 2012 - Elsevier
The chromatographic methods used for the purification of PEGylated proteins are mainly
Size Exclusion (SEC) and Ion Exchange Chromatography (IEX). Although the PEGylation …

Microcalorimetric study of the adsorption of PEGylated lysozyme on a strong cation exchange resin

T Blaschke, J Varon, A Werner, H Hasse - Journal of Chromatography A, 2011 - Elsevier
Adsorption of native as well as mono-, di-, and tri-PEGylated lysozyme on Toyopearl
Gigacap S-650M in sodium phosphate buffer is studied by isothermal titration calorimetry …

Potential application of aqueous two‐phase systems for the fractionation of RNase A and α‐Lactalbumin from their PEGylated conjugates

J González‐Valdez, LF Cueto… - Journal of Chemical …, 2011 - Wiley Online Library
BACKGROUND: PEGylation reactions often result in a heterogeneous population of
conjugated species and unmodified proteins that presents a protein separations challenge …

Aldehyde PEGylation of laccase from Trametes versicolor in route to increase its stability: effect on enzymatic activity

K Mayolo‐Deloisa… - Journal of Molecular …, 2015 - Wiley Online Library
Laccase is a multicopper oxidase that catalyzes the oxidation of phenolic compounds.
Laccase can be used in bioremediation, beverage (wine, fruit juice, and beer) processing …

Experimental study and modeling of the influence of mixed electrolytes on adsorption of macromolecules on a hydrophobic resin

A Werner, H Hasse - Journal of chromatography A, 2013 - Elsevier
The influence of mixed electrolytes on the adsorption of macromolecules on a hydrophobic
resin is explored. The macromolecules are native lysozyme, di-PEG-lysozyme-5kDa and …

Microcalorimetric study of the adsorption of PEGylated lysozyme and PEG on a mildly hydrophobic resin: influence of ammonium sulfate

A Werner, T Blaschke, H Hasse - Langmuir, 2012 - ACS Publications
Adsorption of native as well as mono-, di-, and tri-PEGylated lysozyme on Toyopearl PPG-
600M, a mildly hydrophobic resin is studied by isothermal titration calorimetry and by …

Solubility and binding properties of PEGylated lysozyme derivatives with increasing molecular weight on hydrophobic-interaction chromatographic resins

E Müller, D Josic, T Schröder, A Moosmann - Journal of Chromatography A, 2010 - Elsevier
Dynamic binding capacities and resolution of PEGylated lysozyme derivatives with varying
molecular weights of poly (ethylene) glycol (PEG) with 5kDa, 10kDa and 30kDa for HIC …