Site-specific recombinase systems (Cre-loxP, Flp-FRT, and φC31-att) are transforming both
forward and reverse genetics in mice. By enabling high-fidelity DNA modifications to be …

It has been nearly a decade since the completion of two large-scale chemical mutagenesis
screens in zebrafish, and two years since the completion of a large-scale insertional …

Mouse knockout technology provides a powerful means of elucidating gene function in vivo,
and a publicly available genome-wide collection of mouse knockouts would be significantly …

In the developing brain, transcription factors (TFs) direct the formation of a diverse array of
neurons and glia. We identifed 1445 putative TFs in the mouse genome. We used in situ …

The microRNA-183/96/182 cluster is highly expressed in the retina and other sensory
organs. To uncover its in vivo functions in the retina, we generated a knockout mouse model …

The Hippo kinase pathway plays a central role in growth regulation and tumor suppression
from flies to man. The Hippo/Mst kinase phosphorylates and activates the NDR family kinase …

The mouse is the foremost vertebrate experimental model because its genome can be
precisely and variously engineered. Now that the mouse genome has been sequenced and …

The house mouse has been used as a privileged model organism since the early days of
genetics, and the numerous experiments made with this small mammal have regularly …

Rif1, originally recognized for its role at telomeres in budding yeast, has been implicated in a
wide variety of cellular processes in mammals, including pluripotency of stem cells …

A type of retroviral gene trap vectors has been developed that can induce conditional
mutations in most genes expressed in mouse embryonic stem (ES) cells. The vectors rely on …