Subcellular localization of messenger RNAs (mRNAs) can give precise control over where
protein products are synthesized and operate. However, just 10 years ago many in the …

Intracellular targeting of mRNAs has long been recognized as a means to produce proteins
locally, but has only recently emerged as a prevalent mechanism used by a wide variety of …

Background Cytasters (cytoplasmic asters) are centriole-based nucleation centers of
microtubule polymerization that are observable in large numbers in the cortical cytoplasm of …

Abstract Setting up future body axes is the first important event before and at the beginning
of embryogenesis. The ascidian embryo is a classic model that has been used to gain …

Single-cell RNA sequencing (scRNA-seq) provides a leap forward in resolving cellular
diversity and developmental trajectories but fails to comprehensively delineate the spatial …

Ascidian embryos sequester a specific cytoplasm, called the postplasm, at the posterior
pole, where many maternal RNAs and proteins accumulate. Although the postplasm is …

RNA localization is a widespread mechanism that allows cells to spatially control protein
function by determining their sites of synthesis. In embryos, localized mRNAs are involved in …

The dorsoventral and anteroposterior axes of the ascidian embryo are defined before first
cleavage by means of a series of reorganizations that reposition cytoplasmic and cortical …

During neuronal circuit formation, axons progressively develop into a presynaptic
compartment aided by extracellular signals. Axons display a remarkably high degree of …

Protein translocation into the endoplasmic reticulum (ER) generally requires targeting of
mRNAs encoding secreted or membrane proteins to the ER membrane. The prevalent view …