The plasmid pBR322 was one of the first EK2 multipurpose cloning vectors to be designed
and constructed (ten years ago) for the efficient cloning and selection of recombinant DNA …

* Corresponding author. t Present address: Biotechnology Australia Pty. Ltd., PO Box 20,
Roseville, NSW 2069, Australia. separation of the two plasmid DNA strands, strand transfer …

Chimeric bacterial genes conferring resistance to aminoglycoside antibiotics have been
inserted into the Agrobacterium tumefaciens tumor-inducing (Ti) plasmid and introduced into …

Nucleotide sequence and deletion analysis have been used to identify the regulatory and
coding sequences comprising the cholera toxin operon (ctx). Incorporation of defined in vitro …

Vibrio cholerae strains of the classical biotype all contain two widely separated copies of the
cholera toxin operon ctxAB. In contrast, El Tor strains containing multiple copies of ctx have …

A series of nic− cloning vectors have been constructed analogous to the pUC plasmids but
which are smaller in size and carry more extensive polylinker regions within the lacZ'gene …

A truncated rat neurotensin receptor (NTR), expressed in Escherichia coli with the maltose-
binding protein fused to its N-terminus and the 13 amino acid Bio tag fused to its C-terminus …

We have determined the nucleotide sequence of the sltA and sltB genes that encode the
Shiga-like toxin (SLT) produced by Escherichia coli phage H19B. The amino acid …

The small subunit of iron‐dependent ribonucleotide reductases contains a stable organic
free radical, which is essential for enzyme activity and which is localized to a tyrosine …

Publisher Summary The chapter focuses on the classification and mode of action of the
insecticidal proteins of Bacillus thuringiensis (Bt) or Bt toxin. Bt is a gram-positive, aerobic …