Cryo‐electron microscopy–a primer for the non‐microscopist

JLS Milne, MJ Borgnia, A Bartesaghi… - The FEBS …, 2013 - Wiley Online Library
Cryo‐electron microscopy (cryo‐EM) is increasingly becoming a mainstream technology for
studying the architecture of cells, viruses and protein assemblies at molecular resolution …

Type VI secretion system: secretion by a contractile nanomachine

M Basler - … Transactions of the Royal Society B …, 2015 - royalsocietypublishing.org
The type VI secretion systems (T6SS) are present in about a quarter of all Gram-negative
bacteria. Several key components of T6SS are evolutionarily related to components of …

Type VI secretion requires a dynamic contractile phage tail-like structure

M Basler, M Pilhofer, GP Henderson, GJ Jensen… - Nature, 2012 - nature.com
Type VI secretion systems are bacterial virulence-associated nanomachines composed of
proteins that are evolutionarily related to components of bacteriophage tails. Here we show …

Nitrosopumilus maritimus gen. nov., sp. nov., Nitrosopumilus cobalaminigenes sp. nov., Nitrosopumilus oxyclinae sp. nov., and Nitrosopumilus ureiphilus sp. nov., four marine …

W Qin, KR Heal, R Ramdasi… - … of systematic and …, 2017 - microbiologyresearch.org
Four mesophilic, neutrophilic, and aerobic marine ammonia-oxidizing archaea, designated
strains SCM1T, HCA1T, HCE1T and PS0T, were isolated from a tropical marine fish tank …

Marine tubeworm metamorphosis induced by arrays of bacterial phage tail–like structures

NJ Shikuma, M Pilhofer, GL Weiss, MG Hadfield… - Science, 2014 - science.org
Many benthic marine animal populations are established and maintained by free-swimming
larvae that recognize cues from surface-bound bacteria to settle and metamorphose. Larvae …

Cellular electron cryotomography: toward structural biology in situ

CM Oikonomou, GJ Jensen - Annual review of biochemistry, 2017 - annualreviews.org
Electron cryotomography (ECT) provides three-dimensional views of macromolecular
complexes inside cells in a native frozen–hydrated state. Over the last two decades, ECT …

Assessment of PVA/silver nanocomposite hydrogel patch as antimicrobial dressing scaffold: Synthesis, characterization and biological evaluation

S Bhowmick, V Koul - Materials Science and Engineering: C, 2016 - Elsevier
A novel, elastic, non-adhesive and antimicrobial hydrogel PVA scaffold (loaded with AgNPs)
synthesized using freeze-thaw method has been characterized in this study. The direct …

A new strategy for microbial taxonomic identification through micro‐biosynthetic gold nanoparticles and machine learning

T Yu, S Su, J Hu, J Zhang, Y Xianyu - Advanced Materials, 2022 - Wiley Online Library
Microorganisms can serve as biological factories for the synthesis of inorganic
nanomaterials that can become useful as nanocatalysts, energy‐harvesting–storage …

Electron tomography of cells

L Gan, GJ Jensen - Quarterly reviews of biophysics, 2012 - cambridge.org
The electron microscope has contributed deep insights into biological structure since its
invention nearly 80 years ago. Advances in instrumentation and methodology in recent …

A new view into prokaryotic cell biology from electron cryotomography

CM Oikonomou, YW Chang, GJ Jensen - Nature Reviews Microbiology, 2016 - nature.com
Electron cryotomography (ECT) enables intact cells to be visualized in 3D in an essentially
native state to'macromolecular'(∼ 4 nm) resolution, revealing the basic architectures of …