Gene editing tools: state-of-the-art and the road ahead for the model and non-model fishes

HK Barman, KD Rasal, V Chakrapani, AS Ninawe… - Transgenic …, 2017 - Springer
Advancements in the DNA sequencing technologies and computational biology have
revolutionized genome/transcriptome sequencing of non-model fishes at an affordable cost …

Establishing targeted carp TLR22 gene disruption via homologous recombination using CRISPR/Cas9

V Chakrapani, SK Patra, RP Panda, KD Rasal… - Developmental & …, 2016 - Elsevier
Recent advances in gene editing techniques have not been exploited in farmed fishes. We
established a gene targeting technique, using the CRISPR/Cas9 system in Labeo rohita, a …

First evidence of comparative responses of Toll-like receptor 22 (TLR22) to relatively resistant and susceptible Indian farmed carps to Argulus siamensis infection

RP Panda, V Chakrapani, SK Patra, JN Saha… - Developmental & …, 2014 - Elsevier
Abstract Toll-like receptor 22 (TLR22) is present in teleost but not in mammals. Among
Indian farmed carps, Catla catla is relatively more resistant than Labeo rohita to Argulus …

First evidence of molecular characterization of rohu carp Sox2 gene being expressed in proliferating spermatogonial cells

SK Patra, V Chakrapani, RP Panda, C Mohapatra… - Theriogenology, 2015 - Elsevier
Because little is known about the function of Sox2 (Sry-related box-2) in teleosts, the
objective of this study was to clone and characterize Sox2 complementary DNA (cDNA) from …

Molecular characterization and expression patterns of Nanog gene validating its involvement in the embryonic development and maintenance of spermatogonial stem cells of …

SK Patra, C Vemulawada, MM Soren… - Journal of animal …, 2018 - Springer
Background The homeobox containing transcription factor Nanog plays crucial roles in
embryonic development/proliferation and/or maintenance of spermatogonial stem cells …

[PDF][PDF] Status of transgenic fish production with emphasis on development of food fishes and novel color varieties of ornamental fish: implication and future …

KD Rasal, V Chakrapani, SK Patra… - Journal of …, 2016 - researchgate.net
Transgenesis is the revolutionary science of making a genetically modified organism.
Interestingly, fish offer easier genetic manipulation compared to mammalian counterpart, as …

Genomic organization and hypoxia inducible factor responsive regulation of teleost hsp90β gene during hypoxia stress

HK Barman, SD Mohapatra, V Chakrapani… - Molecular Biology …, 2021 - Springer
Background The physiological significance of a large family of heat-shock proteins (HSPs),
comprised of the cytosolic HSP90A and the endoplasmic reticulum component of HSPB, is …

Identification of promoter within the first intron of Plzf gene expressed in carp spermatogonial stem cells

C Mohapatra, HK Barman - Molecular biology reports, 2014 - Springer
Promyelocytic leukemia zinc finger (Plzf), a transcriptional repressor, is involved in survival
and maintenance of pluripotent stem cells including embryonic and spermatogonial stem …

Gene structure and identification of minimal promoter of Pou2 expressed in spermatogonial cells of rohu carp, Labeo rohita

C Mohapatra, SK Patra, RP Panda, R Mohanta… - Molecular biology …, 2014 - Springer
Mammalian Pou5f1 is a known transcriptional regulator involving maintenance of embryonic
and spermatogonial stem cells. Little is known about teleost Pou2, an ortholog of …

Molecular cloning, characterization and functional assessment of the myosin light polypeptide chain 2 (mylz2) promoter of farmed carp, Labeo rohita

R Mohanta, P Jayasankar, K Das Mahapatra… - Transgenic …, 2014 - Springer
We cloned the 5′-flanking region (1.2 kb) of a muscle-specific gene, encoding myosin light
chain 2 polypeptide (mylz2) of a farmed carp, Labeo rohita (rohu). Sequence analysis using …