The removal of introns from mRNA precursors and its regulation by alternative splicing are
key for eukaryotic gene expression and cellular function, as evidenced by the numerous …

Over the past few decades, RNA sequencing has significantly progressed, becoming a
paramount approach for transcriptome profiling. The revolution from bulk RNA sequencing …

We introduce BacDrop, a highly scalable technology for bacterial single-cell RNA
sequencing that has overcome many challenges hindering the development of scRNA-seq …

Most methods for single-cell transcriptome sequencing amplify the termini of polyadenylated
transcripts, capturing only a small fraction of the total cellular transcriptome. This precludes …

Single-cell analysis is a valuable tool for dissecting cellular heterogeneity in complex
systems. However, a comprehensive single-cell atlas has not been achieved for humans …

The recent maturation of single-cell RNA sequencing (scRNA-seq) technologies has
coincided with transformative new methods to profile genetic, epigenetic, spatial, proteomic …

Patients with systemic lupus erythematosus (SLE) display a complex blood transcriptome
whose cellular origin is poorly resolved. Using single-cell RNA sequencing, we profiled …

Rapid progress in the development of next-generation sequencing (NGS) technologies in
recent years has provided many valuable insights into complex biological systems, ranging …

Single-cell RNA sequencing (scRNA-seq) technologies are poised to reshape the current
cell-type classification system. However, a transcriptome-based single-cell atlas has not …

To facilitate scalable profiling of single cells, we developed split-pool ligation-based
transcriptome sequencing (SPLiT-seq), a single-cell RNA-seq (scRNA-seq) method that …