Imaged capillary isoelectric focusing: applications in the pharmaceutical industry and recent innovations of the technology

J Wu, W McElroy, J Pawliszyn, CD Heger - TrAC Trends in Analytical …, 2022 - Elsevier
Capillary isoelectric focusing with whole-column imaging detection (icIEF) technology is now
the mainstream technology to perform analytical isoelectric focusing analysis in …

Strategies for capillary electrophoresis: method development and validation for pharmaceutical and biological applications—updated and completely revised edition

F Krebs, H Zagst, M Stein, R Ratih, R Minkner… - …, 2023 - Wiley Online Library
This review is in support of the development of selective, precise, fast, and validated
capillary electrophoresis (CE) methods. It follows up a similar article from 1998, Wätzig H …

Capillary isoelectric focusing-mass spectrometry method for the separation and online characterization of intact monoclonal antibody charge variants

J Dai, J Lamp, Q Xia, Y Zhang - Analytical chemistry, 2018 - ACS Publications
We report a new online capillary isoelectric focusing-mass spectrometry (CIEF-MS) method
for monoclonal antibody (mAb) charge variant analysis using an electrokinetically pumped …

Affinity probe capillary electrophoresis: analysis of recombinant human growth hormone with a fluorescent labeled antibody fragment

K Shimura, BL Karger - Analytical chemistry, 1994 - ACS Publications
A new highly sensitive microscaleanalytical procedure called affinity probe capillary
electrophoresis (APCE) ispresented. One of the two species, which can form a biospecific …

Capillary isoelectric focusing

R Rodriguez‐Diaz, T Wehr, M Zhu - Electrophoresis, 1997 - Wiley Online Library
Capillary isoelectric focusing (CIEF) provides excellent resolution of proteins with the
advantage that separations are carried out in a capillary format with on‐tube detection and …

Isoelectric point determination by imaged CIEF of commercially available SARS‐CoV‐2 proteins and the hACE2 receptor

F Krebs, C Scheller, K Grove‐Heike, L Pohl… - …, 2021 - Wiley Online Library
In order to contribute to the scientific research on the severe acute respiratory syndrome
coronavirus 2 (SARS‐CoV‐2), we have investigated the isoelectric points (pI) of several …

Demonstration of isoelectric focusing on an etched quartz chip with UV absorption imaging detection

Q Mao, J Pawliszyn - Analyst, 1999 - pubs.rsc.org
The feasibility of isoelectric focusing (IEF) performed on-chip was demonstrated for the first
time via absorption imaging detection. Microchannels on a quartz chip were fabricated using …

Determination of protein charge variants with (imaged) capillary isoelectric focusing and capillary zone electrophoresis

J Kahle, H Wätzig - Electrophoresis, 2018 - Wiley Online Library
Biopharmaceuticals are increasingly present in pharmaceutical therapy. These highly
complex molecules are still challenging for development and quality control. Charge …

Capillary isoelectric focusing with whole column imaging detection for analysis of proteins and peptides

Q Mao, J Pawliszyn - Journal of biochemical and biophysical methods, 1999 - Elsevier
Whole column imaging detection has been developed for capillary isoelectric focusing
(CIEF) of proteins and peptides. In this imaged CIEF technique, a solution of sample and …

Recent advances in IEF in capillary tubes and microchips

K Shimura - Electrophoresis, 2009 - Wiley Online Library
The methodological advancements in CIEF in tubes and microchips during the period
between 2002 and early 2008 are reviewed as a continuation of previous review by the …