The human gene catalogue is essentially complete, but we lack an equivalently vetted
inventory of bona fide human enhancers. Hundreds of thousands of candidate enhancers …

The pattern of DNA methylation at cytosine bases in the genome is tightly linked to gene
expression, and DNA methylation abnormalities are often observed in diseases. The ten …

Cohesin folds mammalian interphase chromosomes by extruding the chromatin fiber into
numerous loops.“Loop extrusion” can be impeded by chromatin-bound factors, such as …

Cleavage under targets and release using nuclease (CUT&RUN) is an epigenomic profiling
strategy in which antibody-targeted controlled cleavage by micrococcal nuclease releases …

Previously, we described a novel alternative to chromatin immunoprecipitation, CUT&RUN,
in which unfixed permeabilized cells are incubated with antibody, followed by binding of a …

We describe Cleavage Under Targets and Release Using Nuclease (CUT&RUN), a
chromatin profiling strategy in which antibody-targeted controlled cleavage by micrococcal …

Despite an abundance of new studies about topologically associating domains (TADs), the
role of genetic information in TAD formation is still not fully understood. Here we use our …

Background CUT&RUN is an efficient epigenome profiling method that identifies sites of
DNA binding protein enrichment genome-wide with high signal to noise and low sequencing …

Algorithms that accurately predict gene structure from primary sequence alone were
transformative for annotating the human genome. Can we also predict the expression levels …

Cell fate decisions are governed by sequence-specific transcription factors (TFs) that act in
small populations of cells within developing embryos. To understand their functions in vivo, it …