Inverse Molecular Sentinel-Integrated Fiberoptic Sensor for Direct and in Situ Detection of miRNA Targets

P Strobbia, Y Ran, BM Crawford… - Analytical …, 2019 - ACS Publications
Molecular advances have been made in analysis systems for a wide variety of applications
ranging from biodiagnostics, biosafety, bioengineering, and biofuel research applications …

Fast and accurate multiplex identification and quantification of seven genetically modified soybean lines using six-color digital PCR

A Bogožalec Košir, S Muller, J Žel, M Milavec… - Foods, 2023 - mdpi.com
The proliferation of genetically modified organisms (GMOs) presents challenges to GMO
testing laboratories and policymakers. Traditional methods, like quantitative real-time PCR …

Inter-laboratory analysis of selected genetically modified plant reference materials with digital PCR

D Dobnik, T Demšar, I Huber, L Gerdes… - Analytical and …, 2018 - Springer
Digital PCR (dPCR), as a new technology in the field of genetically modified (GM) organism
(GMO) testing, enables determination of absolute target copy numbers. The purpose of our …

Detection of soybean GMO events using two multiplex droplet digital PCR assays

T Demeke, M Eng - Journal of AOAC International, 2024 - academic.oup.com
Background Detection methods for GMO events are required because of regulatory
compliance requirements. Efficient detection and quantification of GMO events saves time …

[PDF][PDF] Guidance document on multiplex real-time PCR methods

L Grohmann, A Barbante… - … Office of the …, 2021 - publications.jrc.ec.europa.eu
Executive Summary Polymerase Chain Reaction (PCR) is considered the gold standard
technique for the detection and quantification of Genetically Modified (GM) food, feed and …

[HTML][HTML] Identification of single target taxon-specific reference assays for the most commonly genetically transformed crops using digital droplet PCR

S Jacchia, DM Kagkli, A Lievens, A Angers-Loustau… - Food Control, 2018 - Elsevier
Abstract Knowledge of the number of DNA sequences targeted by the taxon-specific
reference assays is essential for correct GM quantification and is key to the harmonisation of …

Distribution and degradation of DNA from non-genetically and genetically modified soybean (Roundup Ready): Impact of soybean protein concentrate and soybean …

Y Du, F Chen, G Bu, L Zhang - Food Chemistry, 2021 - Elsevier
To improve genetically modified product labelling legislation and promote the development
of genetically modified foods, the mass variations of genomic DNA and length distributions …

Detection by real-time PCR and pyrosequencing of the cry1Ab and cry1Ac genes introduced in genetically modified (GM) constructs

F Debode, E Janssen, C Bragard… - Food Additives & …, 2017 - Taylor & Francis
The presence of genetically modified organisms (GMOs) in food and feed is mainly detected
by the use of targets focusing on promoters and terminators. As some genes are frequently …

Development of a systematic qPCR array for screening GM soybeans

SB Park, JY Kim, DG Lee, JH Kim, MK Shin, HY Kim - Foods, 2021 - mdpi.com
A screening method using the 35S promoter and nos terminator for genetically modified
organisms (GMOs) is not sufficient to cover all GM soybean events. In this study, a real-time …

Inter-laboratory validation of visual loop-mediated isothermal amplification assays for GM contents screening

R Li, J Shi, B Liu, C Wang, D Zhang, X Zhao, L Yang - Food chemistry, 2019 - Elsevier
Loop-mediated isothermal amplification (LAMP) has been widely used in many fields of
molecular diagnostics, including detection of genetically modified organisms (GMOs) …