Hydroxyl radical protein footprinting (HRPF) coupled to mass spectrometry has been
successfully used to investigate a plethora of protein-related questions. The method, which …

The concept of engineering robust protein scaffolds for novel binding functions emerged 20
years ago, one decade after the advent of recombinant antibody technology. Early examples …

The epidermal growth factor receptor (EGFR) is frequently mutated in human cancer,, and is
an important therapeutic target. EGFR inhibitors have been successful in lung cancer, where …

Antibodies have proved to be a valuable mode of therapy for numerous diseases, mainly
owing to their high target binding affinity and specificity. Unfortunately, antibodies are also …

Non-immunoglobulin (non-Ig) scaffolds are, in contrast to antibodies, small single-domain
proteins that require no post-translational modification, often lack disulfide bonds, and can …

Peptide aptamers are small combinatorial proteins that are selected to bind to specific sites
on their target molecules. Peptide aptamers consist of short, 5-20 amino acid residues long …

Protein detection has wide-ranging implications in molecular diagnostics. Substantial
progress has been made in protein analytics using nanopores and the resistive-pulse …

mRNA display is a powerful biological display platform for the directed evolution of proteins
and peptides. mRNA display libraries covalently link the displayed peptide or protein …

Cysteine-focused chemical proteomic platforms have accelerated the clinical development
of covalent inhibitors for a wide range of targets in cancer. However, how different oncogenic …

Abstract Interleukin (IL-) 23 is a central cytokine controlling TH 17 development.
Overshooting IL-23 signaling contribute to autoimmune diseases. Moreover, GWAS studies …