The 3D structure of chromatin in the nucleus is important for the regulation of gene
expression and the correct deployment of developmental programs. The differentiation of …

To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi
Project (TRiP) at Harvard Medical School (HMS) was established along with several goals …

The zygote has a daunting task ahead of itself; it must develop from a single cell (fertilized
egg) into a fully functioning adult with a multitude of different cell types. In the beginning, the …

Chromatin architecture is fundamental in regulating gene expression. To investigate when
spatial genome organization is first established during development, we examined …

Wolbachia are maternally inherited, intracellular bacteria at the forefront of vector control
efforts to curb arbovirus transmission. In international field trials, the cytoplasmic …

The Drosophila genome activator Vielfaltig (Vfl), also known as Zelda (Zld), is thought to
prime enhancers for activation by patterning transcription factors (TFs). Such priming is …

Summary mRNAs enriched in membraneless condensates provide functional
compartmentalization within cells. The mechanisms that recruit transcripts to condensates …

The evaluation of specific endogenous transcript levels is important for understanding
transcriptional regulation. More specifically, it is useful for independent confirmation of …

Developmental enhancers integrate graded concentrations of transcription factors (TFs) to
create sharp gene expression boundaries. Here we examine the hunchback P2 (HbP2) …

Kinesin-1 carries cargos including proteins, RNAs, vesicles, and pathogens over long
distances within cells. The mechanochemical cycle of kinesins is well described, but how …