The emerging and uncultivated potential of CRISPR technology in plant science
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
Next-generation breeding strategies for climate-ready crops
Climate change is a threat to global food security due to the reduction of crop productivity
around the globe. Food security is a matter of concern for stakeholders and policymakers as …
around the globe. Food security is a matter of concern for stakeholders and policymakers as …
Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems
Y Zhang, Q Ren, X Tang, S Liu, AA Malzahn… - Nature …, 2021 - nature.com
CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …
Optimization of AsCas12a for combinatorial genetic screens in human cells
Cas12a RNA-guided endonucleases are promising tools for multiplexed genetic
perturbations because they can process multiple guide RNAs expressed as a single …
perturbations because they can process multiple guide RNAs expressed as a single …
CRISPR-Cas-mediated transcriptional modulation: The therapeutic promises of CRISPRa and CRISPRi
L Bendixen, TI Jensen, RO Bak - Molecular Therapy, 2023 - cell.com
The CRISPR-Cas system is commonly known for its ability to cleave DNA in a
programmable manner, which has democratized gene editing and facilitated recent …
programmable manner, which has democratized gene editing and facilitated recent …
CRISPR-Cas12a (Cpf1): a versatile tool in the plant genome editing tool box for agricultural advancement
A Bandyopadhyay, N Kancharla, VS Javalkote… - Frontiers in plant …, 2020 - frontiersin.org
Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion
from today. To meet the demands of growing, geographically and socio-economically …
from today. To meet the demands of growing, geographically and socio-economically …
Expanding the CRISPR toolbox in zebrafish for studying development and disease
The study of model organisms has revolutionized our understanding of the mechanisms
underlying normal development, adult homeostasis, and human disease. Much of what we …
underlying normal development, adult homeostasis, and human disease. Much of what we …
Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases
The widespread use of Cas12a (formerly Cpf1) nucleases for genome engineering is limited
by their requirement for a rather long TTTV protospacer adjacent motif (PAM) sequence …
by their requirement for a rather long TTTV protospacer adjacent motif (PAM) sequence …
Exploration of plant-microbe interactions for sustainable agriculture in CRISPR era
Plants and microbes are co-evolved and interact with each other in nature. Plant-associated
microbes, often referred to as plant microbiota, are an integral part of plant life. Depending …
microbes, often referred to as plant microbiota, are an integral part of plant life. Depending …
Conformational dynamics of CasX (Cas12e) in mediating DNA cleavage revealed by single-molecule FRET
W Xing, D Li, W Wang, JJG Liu… - Nucleic Acids Research, 2024 - academic.oup.com
CasX (also known as Cas12e), a Class 2 CRISPR-Cas system, shows promise in genome
editing due to its smaller size compared to the widely used Cas9 and Cas12a. Although the …
editing due to its smaller size compared to the widely used Cas9 and Cas12a. Although the …