The stability of proteins in an aqueous solution can be modified by the presence of
osmolytes. The hydration sphere of stabilizing osmolytes is strikingly similar to the enhanced …

Kramers rate theory is a milestone in chemical reaction research, but concerns regarding the
basic understanding of condensed phase reaction rates of large molecules in viscous milieu …

Cytochrome c (cyt c) is a small globular hemoprotein with the main function as an electron
carrier in mitochondrial respiratory chain. Cyt c possesses also peroxidase-like activity in the …

This article describes the molecular mechanism by which tetraalkylammonium chloride ([R 4
N] Cl: R-= methyl (Me), ethyl (Et), propyl (Pr), butyl (Bu)) modulates the stability, folding, and …

We analyzed the hydration behavior of alkyl-substituted urea osmolytes in terms of their
deviation from Lewis–Randall solution ideality and characterized their structure …

Imaged capillary isoelectric focusing (icIEF) separates ampholytic components of
biomolecules in an electric field according to their isoelectric points and has been used for …

Capillary isoelectric focusing (cIEF) is normally run under denaturing conditions using urea
to expose any buried protein residues that may contribute to the overall charge. However …

This paper analyzes the effect of pH on thermodynamic stability and folding kinetics of horse
cytochrome c (cyt c). Analysis of equilibrium unfolding transitions of Ferricyt c and Ferrocyt c …

This manuscript determines the factors by which the water-miscible ionic liquid (IL), 1-alkyl-3-
methylimidazolium chloride [Rmim] Cl affects the thermodynamic stability and CO …

A compatible osmolyte such as glycine betaine (GB) and low concentrations of a denaturant
constrain the internal dynamics of natively folded carbonmonoxycytochrome c (NCO) at pH …