Advanced strategies and tools to facilitate and streamline microbial adaptive laboratory evolution

Y Wu, A Jameel, XH Xing, C Zhang - Trends in biotechnology, 2022 - cell.com
Adaptive laboratory evolution (ALE) has served as a historic microbial engineering method
that mimics natural selection to obtain desired microbes. The past decade has witnessed …

Recent advances toward the bioconversion of methane and methanol in synthetic methylotrophs

GJ Gregory, RK Bennett, ET Papoutsakis - Metabolic Engineering, 2022 - Elsevier
Abundant natural gas reserves, along with increased biogas production, have prompted
recent interest in harnessing methane as an industrial feedstock for the production of liquid …

C1-based biomanufacturing: Advances, challenges and perspectives

X Lv, W Yu, C Zhang, P Ning, J Li, Y Liu, G Du… - Bioresource …, 2023 - Elsevier
Abstract One-carbon (C1) compounds have emerged as a key research focus due to the
growth of metabolic engineering and synthetic biology as affordable and sustainable …

Adaptive laboratory evolution of microorganisms: Methodology and application for bioproduction

T Hirasawa, T Maeda - Microorganisms, 2022 - mdpi.com
Adaptive laboratory evolution (ALE) is a useful experimental methodology for fundamental
scientific research and industrial applications to create microbial cell factories. By using ALE …

Engineered Corynebacterium glutamicum as the Platform for the Production of Aromatic Aldehydes

HS Kim, JA Choi, BY Kim, L Ferrer, JM Choi… - … in Bioengineering and …, 2022 - frontiersin.org
Aromatic aldehydes, including 4-hydroxybenzaldehyde (4-HB aldehyde), protocatechuic
(PC) aldehyde, and vanillin, are used as important flavors, fragrances, and pharmaceutical …

Unravelling formaldehyde metabolism in bacteria: road towards synthetic methylotrophy

VJ Klein, M Irla, M Gil López, T Brautaset… - Microorganisms, 2022 - mdpi.com
Formaldehyde metabolism is prevalent in all organisms, where the accumulation of
formaldehyde can be prevented through the activity of dissimilation pathways. Furthermore …

Rewiring the native methanol assimilation metabolism by incorporating the heterologous ribulose monophosphate cycle into Methylorubrum extorquens

XJ Yuan, WJ Chen, ZX Ma, QQ Yuan, M Zhang… - Metabolic …, 2021 - Elsevier
Methanol is assimilated through the serine cycle to generate acetyl-CoA without carbon loss.
However, a highly active serine cycle requires high consumption of reducing equivalents …

Methanol dehydrogenases as a key biocatalysts for synthetic methylotrophy

TK Le, YJ Lee, GH Han, SJ Yeom - Frontiers in Bioengineering and …, 2021 - frontiersin.org
One-carbon (C1) chemicals are potential building blocks for cheap and sustainable re-
sources such as methane, methanol, formaldehyde, formate, carbon monoxide, and more …

Adaptive laboratory evolution accelerated glutarate production by Corynebacterium glutamicum

C Prell, T Busche, C Rückert, L Nolte… - Microbial Cell …, 2021 - Springer
Background The demand for biobased polymers is increasing steadily worldwide. Microbial
hosts for production of their monomeric precursors such as glutarate are developed. To meet …

Developing methylotrophic microbial platforms for a methanol-based bioindustry

HB Singh, MK Kang, M Kwon, SW Kim - Frontiers in Bioengineering …, 2022 - frontiersin.org
Methanol, a relatively cheap and renewable single-carbon feedstock, has gained
considerable attention as a substrate for the bio-production of commodity chemicals …