Mutagenesis of proteins is of paramount importance chain reaction (PCR) or non-PCR, has
revolutionized the means by which mutants are obtained (2, 3). Mutafor understanding the …

The polymerase chain reaction (PCR), first described by Saiki et al.(79), is a highly sensitive
and specific methodology for detection of nucleic acids and a useful tool for quantitation of …

Posttranslational modification can confer special functions to peptides. Based on exhaustive
liquid chromatography mass spectrometry analysis targeting tyrosine-sulfated peptides, we …

PCR ligation mutagenesis is a novel technique that can easily be adapted for many gene
modification purposes. Successful application of this versatile technique involves sequence …

Mariner-family transposable elements are active in a wide variety of organisms and are
becoming increasingly important genetic tools in species lacking sophisticated genetics. The …

Protein-protein interactions (PPIs) play crucial roles in various biological processes. Among
biochemical, genetic, and imaging approaches that have been used for the study of PPIs …

Splice site consensus sequences alone are insufficient to dictate the recognition of real
constitutive splice sites within the typically large transcripts of higher eukaryotes, and large …

Mariner family transposable elements are widespread in animals, but their regulation is
poorly understood, partly because only two are known to be functional. These are particular …

Secretion of effector proteins in enteropathogenic Escherichia coli (EPEC) is mediated by a
specialized type III secretion system whose components are encoded in the LEE1, LEE2 …

Cyclin-dependent kinase inhibitors (cdkis), such as p21, are believed to control proliferation
through an ability to function as stoichiometric antagonists of cyclin-dependent kinases …