Advancing point-of-care microbial pathogens detection by material-functionalized microfluidic systems
Background Public health events caused by foodborne pathogens have brought substantial
economic and social burdens. Conventional pathogen detection relies on a time-consuming …
economic and social burdens. Conventional pathogen detection relies on a time-consuming …
[HTML][HTML] Fly Me to the Micron: Microtechnologies for Drosophila Research
Multicellular model organisms, such as Drosophila melanogaster (fruit fly), are frequently
used in a myriad of biological research studies due to their biological significance and …
used in a myriad of biological research studies due to their biological significance and …
[HTML][HTML] Incorporating the image formation process into deep learning improves network performance
Abstract We present Richardson–Lucy network (RLN), a fast and lightweight deep learning
method for three-dimensional fluorescence microscopy deconvolution. RLN combines the …
method for three-dimensional fluorescence microscopy deconvolution. RLN combines the …
Multiview confocal super-resolution microscopy
Confocal microscopy remains a major workhorse in biomedical optical microscopy owing to
its reliability and flexibility in imaging various samples, but suffers from substantial point …
its reliability and flexibility in imaging various samples, but suffers from substantial point …
Large-scale high-throughput 3D culture, imaging, and analysis of cell spheroids using microchip-enhanced light-sheet microscopy
Light sheet microscopy combined with a microchip is an emerging tool in biomedical
research that notably improves efficiency. However, microchip-enhanced light-sheet …
research that notably improves efficiency. However, microchip-enhanced light-sheet …
[HTML][HTML] 3D live imaging and phenotyping of CAR-T cell mediated-cytotoxicity using high-throughput Bessel oblique plane microscopy
Z Wang, J Wang, Y Zhao, J Jin, W Si, L Chen… - Nature …, 2024 - nature.com
Clarification of the cytotoxic function of T cells is crucial for understanding human immune
responses and immunotherapy procedures. Here, we report a high-throughput Bessel …
responses and immunotherapy procedures. Here, we report a high-throughput Bessel …
Deep-learning on-chip light-sheet microscopy enabling video-rate volumetric imaging of dynamic biological specimens
X Chen, J Ping, Y Sun, C Yi, S Liu, Z Gong, P Fei - Lab on a Chip, 2021 - pubs.rsc.org
Volumetric imaging of dynamic signals in a large, moving, and light-scattering specimen is
extremely challenging, owing to the requirement on high spatiotemporal resolution and …
extremely challenging, owing to the requirement on high spatiotemporal resolution and …
A multi-functional microfluidic device compatible with widefield and light sheet microscopy
We present a microfluidic device compatible with high resolution light sheet and super-
resolution microscopy. The device is a 150 μm thick chamber with a transparent fluorinated …
resolution microscopy. The device is a 150 μm thick chamber with a transparent fluorinated …
[HTML][HTML] Volumetric imaging of human mesenchymal stem cells (hMSCs) for non-destructive quantification of 3D cell culture growth
The adoption of cell-based therapies into the clinic will require tremendous large-scale
expansion to satisfy future demand, and bioreactor-microcarrier cultures are best suited to …
expansion to satisfy future demand, and bioreactor-microcarrier cultures are best suited to …
[HTML][HTML] A light sheet fluorescence microscopy protocol for Caenorhabditis elegans larvae and adults
Light sheet fluorescence microscopy (LSFM) has become a method of choice for live
imaging because of its fast acquisition and reduced photobleaching and phototoxicity …
imaging because of its fast acquisition and reduced photobleaching and phototoxicity …