Recent developments in bioprocessing of recombinant proteins: expression hosts and process development

NK Tripathi, A Shrivastava - Frontiers in Bioengineering and …, 2019 - frontiersin.org
Infectious diseases, along with cancers, are among the main causes of death among
humans worldwide. The production of therapeutic proteins for treating diseases at large …

Cell culture media for recombinant protein expression in Chinese hamster ovary (CHO) cells: History, key components, and optimization strategies

FV Ritacco, Y Wu, A Khetan - Biotechnology progress, 2018 - Wiley Online Library
The culture of Chinese Hamster Ovary (CHO) cells for modern industrial applications, such
as expression of recombinant proteins, requires media that support growth and production …

Spontaneous immortalization of chicken fibroblasts generates stable, high-yield cell lines for serum-free production of cultured meat

L Pasitka, M Cohen, A Ehrlich, B Gildor, E Reuveni… - Nature Food, 2023 - nature.com
Cellular agriculture could meet growing demand for animal products, but yields are typically
low and regulatory bodies restrict genetic modification for cultured meat production. Here we …

Large-scale cultured meat production: Trends, challenges and promising biomanufacturing technologies

L Chen, D Guttieres, A Koenigsberg, PW Barone… - Biomaterials, 2022 - Elsevier
Food systems of the future will need to face an increasingly clear reality–that a protein-rich
diet is essential for good health, but traditional meat products will not suffice to ensure safety …

Scale‐up economics for cultured meat

D Humbird - Biotechnology and Bioengineering, 2021 - Wiley Online Library
This analysis examines the potential of “cultured meat” products made from edible animal
cell culture to measurably displace the global consumption of conventional meat …

Trends in upstream and downstream process development for antibody manufacturing

P Gronemeyer, R Ditz, J Strube - Bioengineering, 2014 - mdpi.com
A steady increase of product titers and the corresponding change in impurity composition
represent a challenge for development and optimization of antibody production processes …

Continuous downstream processing for high value biological products: a review

AL Zydney - Biotechnology and bioengineering, 2016 - Wiley Online Library
There is growing interest in the possibility of developing truly continuous processes for the
large‐scale production of high value biological products. Continuous processing has the …

[HTML][HTML] Apoptosis: A mammalian cell bioprocessing perspective

AL Grilo, A Mantalaris - Biotechnology advances, 2019 - Elsevier
Apoptosis is a form of programmed and controlled cell death that accounts for the majority of
cellular death in bioprocesses. Cell death affects culture longevity and product quality; it is …

Bioprocess design considerations for cultured meat production with a focus on the expansion bioreactor

SJ Allan, PA De Bank, MJ Ellis - Frontiers in Sustainable Food …, 2019 - frontiersin.org
Cultured meat, as a cellular agriculture product, utilizes tissue engineering techniques and
consequently faces not only cell culture challenges but also scale-up limitations. To ensure …

Optimization of protein expression in mammalian cells

M Hunter, P Yuan, D Vavilala… - Current protocols in …, 2019 - Wiley Online Library
Recombinant proteins, such as monoclonal antibodies, are produced in mammalian cell
lines to introduce proper protein folding and post‐translational modifications, which are …