Transcription-factor-based biosensor engineering for applications in synthetic biology

N Ding, S Zhou, Y Deng - ACS Synthetic Biology, 2021 - ACS Publications
Transcription-factor-based biosensors (TFBs) are often used for metabolite detection,
adaptive evolution, and metabolic flux control. However, designing TFBs with superior …

Applications of synthetic biology in medical and pharmaceutical fields

X Yan, X Liu, C Zhao, GQ Chen - Signal transduction and targeted …, 2023 - nature.com
Synthetic biology aims to design or assemble existing bioparts or bio-components for useful
bioproperties. During the past decades, progresses have been made to build delicate …

The all-E. coliTXTL toolbox 3.0: new capabilities of a cell-free synthetic biology platform

D Garenne, S Thompson, A Brisson… - Synthetic …, 2021 - academic.oup.com
The new generation of cell-free gene expression systems enables the prototyping and
engineering of biological systems in vitro over a remarkable scope of applications and …

A comprehensive review on the applications of nano-biosensor-based approaches for non-communicable and communicable disease detection

RG Kerry, KE Ukhurebor, S Kumari, GK Maurya… - Biomaterials …, 2021 - pubs.rsc.org
The outstretched applications of biosensors in diverse domains has become the reason for
their attraction for scientific communities. Because they are analytical devices, they can …

Synthetic cells in biomedical applications

W Sato, T Zajkowski, F Moser… - Wiley Interdisciplinary …, 2022 - Wiley Online Library
Synthetic cells are engineered vesicles that can mimic one or more salient features of life.
These features include directed localization, sense‐and‐respond behavior, gene …

Advances in riboswitch‐based biosensor as food samples detection tool

X Dong, S Qi, IM Khan, Y Sun… - … Reviews in Food …, 2023 - Wiley Online Library
Food safety has always been a hot issue of social concern, and biosensing has been widely
used in the field of food safety detection. Compared with traditional aptamer‐based …

Biotechnology applications of cell-free expression systems

A Brookwell, JP Oza, F Caschera - Life, 2021 - mdpi.com
Cell-free systems are a rapidly expanding platform technology with an important role in the
engineering of biological systems. The key advantages that drive their broad adoption are …

Development of Cas12a-based cell-free small-molecule biosensors via allosteric regulation of CRISPR array expression

A Mahas, Q Wang, T Marsic, MM Mahfouz - Analytical chemistry, 2022 - ACS Publications
Cell-free biosensors can detect various molecules, thus promising to transform the
landscape of diagnostics. Here, we developed a simple, rapid, sensitive, and field …

[HTML][HTML] Towards detection of SARS-CoV-2 RNA in human saliva: a paper-based cell-free toehold switch biosensor with a visual bioluminescent output

JP Hunt, EL Zhao, TJ Free, M Soltani, CA Warr… - New …, 2022 - Elsevier
The COVID-19 pandemic has illustrated the global demand for rapid, low-cost, widely
distributable and point-of-care nucleic acid diagnostic technologies. Such technologies …

Detection of viral RNAs at ambient temperature via reporter proteins produced through the target-splinted ligation of DNA probes

EA Phillips, AD Silverman, A Joneja, M Liu… - Nature Biomedical …, 2023 - nature.com
Nucleic acid assays are not typically deployable in point-of-care settings because they
require costly and sophisticated equipment for the control of the reaction temperature and for …