Quantitative real-time PCR approaches for microbial community studies in wastewater treatment systems: applications and considerations

J Kim, J Lim, C Lee - Biotechnology advances, 2013 - Elsevier
Quantitative real-time PCR (qPCR) has been widely used in recent environmental microbial
ecology studies as a tool for detecting and quantifying microorganisms of interest, which …

Responses of microbial activity, abundance, and community in wheat soil after three years of heavy fertilization with manure-based compost and inorganic nitrogen

W Tian, L Wang, Y Li, K Zhuang, G Li, J Zhang… - Agriculture, Ecosystems …, 2015 - Elsevier
A three-year field experiment in a winter wheat–rice cropping system was performed to
compare the influence on soil microbial parameters including microbial activity, abundance …

Unravelling the reasons for disproportion in the ratio of AOB and NOB in aerobic granular sludge

MKH Winkler, JP Bassin, R Kleerebezem… - Applied Microbiology …, 2012 - Springer
In this study, we analysed the nitrifying microbial community (ammonium-oxidizing bacteria
(AOB) and nitrite-oxidizing bacteria (NOB)) within three different aerobic granular sludge …

Partitioning of nutrient removal contribution between granules and flocs in a hybrid granular activated sludge system

SP Wei, HD Stensel, RM Ziels, S Herrera, PH Lee… - Water Research, 2021 - Elsevier
Sludge granulation in continuous-flow systems is an emerging technology to intensify
existing activated sludge infrastructure for nutrient removal. In these systems, the nutrient …

Application of a moving bed biofilm reactor for tertiary ammonia treatment in high temperature industrial wastewater

JL Shore, WS M'Coy, CK Gunsch, MA Deshusses - Bioresource technology, 2012 - Elsevier
This study examines the use of a moving bed biofilm reactor (MBBR) as a tertiary treatment
step for ammonia removal in high temperature (35–45° C) effluents, and quantifies different …

Evaluating the solid retention time of bacteria in flocculent and granular sludge

MKH Winkler, R Kleerebezem, WO Khunjar, B de Bruin… - Water research, 2012 - Elsevier
The specific solid retention time for different bacteria within flocculent and granular sludge
was determined. Samples were collected from reactor and effluent sludge and the number of …

Stochastic processes govern invasion success in microbial communities when the invader is phylogenetically close to resident bacteria

M Kinnunen, A Dechesne, HJ Albrechtsen… - The ISME …, 2018 - academic.oup.com
Despite recent efforts in identifying the determinants of invasion in microbial communities,
experimental observations across different ecosystems are inconclusive. While relationships …

Changes in the actinomycetal communities during continuous thermophilic composting as revealed by denaturing gradient gel electrophoresis and quantitative PCR

Y Xiao, GM Zeng, ZH Yang, YH Ma, C Huang… - Bioresource …, 2011 - Elsevier
Actinomycetes degrade cellulose and solubilize lignin during composting. Changes in the
diversity of the actinomycetal communities and the 16S rDNA copy numbers of …

Diazotrophic diversity, nifH gene expression and nitrogenase activity in a rice paddy field in Fujian, China

L Mårtensson, B Díez, I Wartiainen, W Zheng… - Plant and Soil, 2009 - Springer
The diazotrophic communities in a rice paddy field were characterized by a molecular
polyphasic approach including DNA/RNA-DGGE fingerprinting, real time RT-PCR analysis …

Metagenomic analysis and microbial activity shifts reveal the effect of the carbon/nitrogen ratio on the nitrogen removal performance of a moving bed biofilm reactor …

Q Wang, S Wu, GY Chu, Z Zhang, Z She, Y Zhao… - Journal of Water …, 2023 - Elsevier
The performance evaluation, metagenomic analysis, and microbial activity of a moving bed
biofilm reactor (MBBR) were performed in treating mariculture wastewater at different …