RNA granules: functional compartments or incidental condensates?

A Putnam, L Thomas, G Seydoux - Genes & development, 2023 - genesdev.cshlp.org
RNA granules are mesoscale assemblies that form in the absence of limiting membranes.
RNA granules contain factors for RNA biogenesis and turnover and are often assumed to …

Biosynthetic pathway and metabolic engineering of succinic acid

X Liu, G Zhao, S Sun, C Fan, X Feng… - … in Bioengineering and …, 2022 - frontiersin.org
Succinic acid, a dicarboxylic acid produced as an intermediate of the tricarboxylic acid (TCA)
cycle, is one of the most important platform chemicals for the production of various high …

Choline chloride pretreatment on volatile fatty acids promotion from sludge anaerobic fermentation: in-situ deep eutectic solvents-like formation for EPS disintegration …

W Cao, S Fang, Q Wu, Y Wu, L Feng, Z Xie… - Chemical Engineering …, 2023 - Elsevier
Slow solubilization/hydrolysis efficiency has been widely recognized as a limiting factor for
volatile fatty acids (VFAs) production from waste activated sludge (WAS) via anaerobic …

Heterotypic phase separation of Hfq is linked to its roles as an RNA chaperone

O Goldberger, T Szoke, A Nussbaum-Shochat… - Cell Reports, 2022 - cell.com
Hfq, an Sm-like protein and the major RNA chaperone in E. coli, has been shown to
distribute non-uniformly along a helical path under normal growth conditions and to relocate …

Phase-separated biomolecular condensates for biocatalysis

S Lim, DS Clark - Trends in Biotechnology, 2024 - cell.com
Nature often uses dynamically assembling multienzymatic complexes called metabolons to
achieve spatiotemporal control of complex metabolic reactions. Researchers are aiming to …

Photo‐Responsive Phase‐Separating Fluorescent Molecules for Intracellular Protein Delivery

Y Bao, H Chen, Z Xu, J Gao, L Jiang… - Angewandte Chemie, 2023 - Wiley Online Library
Cellular membranes, including the plasma and endosome membranes, are barriers to
outside proteins. Various vehicles have been devised to deliver proteins across the plasma …

[HTML][HTML] Synergetic engineering of Escherichia coli for efficient production of L-tyrosine

J Ping, L Wang, Z Qin, Z Zhou, J Zhou - Synthetic and Systems …, 2023 - Elsevier
Abstract l-Tyrosine, an aromatic non-essential amino acid, is the raw material for many
important chemical products, including levodopa, resveratrol, and hydroxytyrosol. It is widely …

Liquid–Liquid Phase Separation and protective protein aggregates in bacteria

D Kuczyńska-Wiśnik, K Stojowska-Swędrzyńska… - Molecules, 2023 - mdpi.com
Liquid–liquid phase separation (LLPS) and the formation of membraneless organelles
(MLOs) contribute to the spatiotemporal organization of various physiological processes in …

Enhanced precision and efficiency in metabolic regulation: compartmentalized metabolic engineering

R Wang, Y Su, W Yang, H Zhang, J Wang… - Bioresource Technology, 2024 - Elsevier
Metabolic engineering has witnessed remarkable advancements, enabling successful large-
scale, cost-effective and efficient production of numerous compounds. However, the …

Cell Surface Engineering by Phase‐Separated Coacervates for Antibody Display and Targeted Cancer Cell Therapy

H Chen, Y Bao, X Li, F Chen, R Sugimura… - Angewandte …, 2024 - Wiley Online Library
Cell therapies such as CAR− T have demonstrated significant clinical successes, driving the
investigation of immune cell surface engineering using natural and synthetic materials to …