Considered by some to be among the simpler forms of life, viruses represent highly evolved
natural vectors for the transfer of foreign genetic information into cells. This attribute has led …

Herpes simplex viruses (HSV) type 1 and type 2 are responsible for recurrent orolabial and
genital infections. The standard therapy for the management of HSV infections includes …

Gene therapy can be broadly defined as the transfer of genetic material to cure a disease or
at least to improve the clinical status of a patient. One of the basic concepts of gene therapy …

We have recently introduced a novel procedure for the construction of herpesvirus mutants
that is based on the cloning and mutagenesis of herpesvirus genomes as infectious …

Gammaherpesviruses cause important infections of humans, in particular in
immunocompromised patients. Recently, murine gammaherpesvirus 68 (MHV-68) infection …

A key factor in the success of gene therapy is the development of gene delivery systems that
are capable of efficient gene transfer in a broad variety of tissues, without causing any …

In recent years, several laboratories have reported on the cloning of herpes simplex virus
type 1 (HSV-1) genomes as bacterial artificial chromosomes (BACs) in Escherichia coli and …

We report that herpes simplex virus 1 (HSV-1) infection can activate and exploit a cellular
DNA damage response that aids viral replication in nonneuronal cells. Early in HSV-1 …

The full-length genome of human cytomegalovirus strain AD169 was cloned as an infectious
bacterial artificial chromosome (BAC) plasmid, pAD/Cre. The BAC vector, flanked by LoxP …

We have cloned the human cytomegalovirus (HCMV) genome as an infectious bacterial
artificial chromosome (BAC) in Escherichia coli. Here, we have subjected the HCMV BAC to …