To inform management and conservation decisions, environmental DNA (eDNA) methods
are used to detect genetic material shed into the water by imperiled and invasive species …

Recent taxonomic studies on fungi using molecular techniques have provided important
information on their phylogenies and new classifications (Chapter VI). Many genera of …

The use of real time quantitative PCR (qPCR) has recently been extended to food science.
The literature has mainly focused on its use in ensuring food safety. However, it offers a …

Bacterial diversity in soil is high relative to more homogeneous environments (eg, freshwater
or marine habitats). Isolation imparted by fragmented aquatic microhabitats in unsaturated …

Quantitative real‐time PCR (qPCR) has been widely used in quantifying bacterial and fungal
populations in various ecosystems, as well as the fungi to bacteria ratio (F: B ratio). Recently …

Real-time PCR for the quantitative assessment of microbial genes in DNA extracted from
environmental samples is increasingly being used in microbial ecology studies. A significant …

Because of the increased population density, high-risk behavior of young students, and
lower vaccination rates, university campuses are considered hot spots for COVID-19 …

Quantitative real-time PCR (qPCR) analysis for accurate quantification of targeted microbial
genes is compromised by the presence of co-extracted inhibitors from soil samples. Dilution …

The few studies attempting to specifically characterize dermatophytes from hair samples of
dogs and cats using PCR-based methodology relied on sequence-based analysis of …

Nucleic acid extraction from complex environmental and ancient tissue material is prone to
co‐extract inhibitory substances that make further molecular analysis difficult or impossible …