Kinetic mechanism of DNA polymerases: contributions of conformational dynamics and a third divalent metal ion
Faithful transmission and maintenance of genetic material is primarily fulfilled by DNA
polymerases. During DNA replication, these enzymes catalyze incorporation of …
polymerases. During DNA replication, these enzymes catalyze incorporation of …
Physics of biomolecular recognition and conformational dynamics
Biomolecular recognition usually leads to the formation of binding complexes, often
accompanied by large-scale conformational changes. This process is fundamental to …
accompanied by large-scale conformational changes. This process is fundamental to …
Multiple deprotonation paths of the nucleophile 3′-OH in the DNA synthesis reaction
DNA synthesis by polymerases is essential for life. Deprotonation of the nucleophile 3′-OH
is thought to be the obligatory first step in the DNA synthesis reaction. We have examined …
is thought to be the obligatory first step in the DNA synthesis reaction. We have examined …
Structural insights into the post-chemistry steps of nucleotide incorporation catalyzed by a DNA polymerase
DNA polymerases are essential enzymes that faithfully and efficiently replicate genomic
information. 1− 3 The mechanism of nucleotide incorporation by DNA polymerases has …
information. 1− 3 The mechanism of nucleotide incorporation by DNA polymerases has …
Investigating the conformational dynamics of a Y-family DNA polymerase during its folding and binding to DNA and a nucleotide
During DNA polymerization, the Y-family DNA polymerases are capable of bypassing
various DNA damage, which can stall the replication fork progression. It has been well …
various DNA damage, which can stall the replication fork progression. It has been well …
In crystallo observation of three metal ion promoted DNA polymerase misincorporation
C Chang, C Lee Luo, Y Gao - Nature Communications, 2022 - nature.com
Error-free replication of DNA is essential for life. Despite the proofreading capability of
several polymerases, intrinsic polymerase fidelity is in general much higher than what base …
several polymerases, intrinsic polymerase fidelity is in general much higher than what base …
Conformational Dynamics of DNA Polymerases Revealed at the Single-Molecule Level
DP Millar - Frontiers in Molecular Biosciences, 2022 - frontiersin.org
DNA polymerases are intrinsically dynamic macromolecular machines. The purpose of this
review is to describe the single-molecule Förster resonance energy transfer (smFRET) …
review is to describe the single-molecule Förster resonance energy transfer (smFRET) …
Investigating the trade-off between folding and function in a multidomain Y-family DNA polymerase
The way in which multidomain proteins fold has been a puzzling question for decades. Until
now, the mechanisms and functions of domain interactions involved in multidomain protein …
now, the mechanisms and functions of domain interactions involved in multidomain protein …
[HTML][HTML] In crystallo observation of active site dynamics and transient metal ion binding within DNA polymerases
C Chang, G Zhou, Y Gao - Structural Dynamics, 2023 - pubs.aip.org
DNA polymerases are the enzymatic catalysts that synthesize DNA during DNA replication
and repair. Kinetic studies and x-ray crystallography have uncovered the overall kinetic …
and repair. Kinetic studies and x-ray crystallography have uncovered the overall kinetic …
Advances in Structural and Single-Molecule Methods for Investigating DNA Lesion Bypass and Repair Polymerases
Innovative advances in X-ray crystallography and single-molecule biophysics have yielded
unprecedented insight into the mechanisms of DNA lesion bypass and damage repair. Time …
unprecedented insight into the mechanisms of DNA lesion bypass and damage repair. Time …