The discovery and design of biologically important RNA molecules is outpacing three-
dimensional structural characterization. Here, we demonstrate that cryo-electron microscopy …

Recent technical advances have dramatically increased the power and scope of structural
biology. New developments in high-resolution cryo-electron microscopy, serial X-ray …

Single particle cryo-electron microscopy (cryo-EM) has emerged as the prevailing method
for near-atomic structure determination, shedding light on the important molecular …

We report a fully automated procedure for the optimization and interpretation of
reconstructions from cryo-electron microscopy (cryo-EM) data, available in Phenix as …

Cytidine triphosphate synthase (CTPS), which comprises an ammonia ligase domain and a
glutamine amidotransferase domain, catalyzes the final step of de novo CTP biosynthesis …

Intracellular compartmentation is a key strategy for the functioning of a cell. In 2010, several
studies revealed that the metabolic enzyme CTP synthase (CTPS) can form filamentous …

Increasingly, cryo-electron microscopy (cryo-EM) is used to determine the structures of RNA–
protein assemblies, but nearly all maps determined with this method have biologically …

Advances in cryoelectron microscopy (cryo-EM) have revolutionized the structural
investigation of large macromolecular assemblies. In this review, we first provide a broad …

A procedure for building protein chains into maps produced by single‐particle electron cryo‐
microscopy (cryo‐EM) is described. The procedure is similar to the way an experienced …

The resolution of electron-potential maps in single-particle cryo-electron microscopy
(cryoEM) is approaching atomic or near-atomic resolution. However, no program currently …