Seed targeted RNAi-mediated silencing of GmMIPS1 limits phytate accumulation and improves mineral bioavailability in soybean

A Kumar, V Kumar, V Krishnan, A Hada, A Marathe… - Scientific reports, 2019 - nature.com
Phytic acid (PA), the major phosphorus reserve in soybean seeds (60–80%), is a potent ion
chelator, causing deficiencies that leads to malnutrition. Several forward and reverse …

Improved Agrobacterium tumefaciens-mediated transformation of soybean [Glycine max (L.) Merr.] following optimization of culture conditions and mechanical …

A Hada, V Krishnan, MS Mohamed Jaabir… - In Vitro Cellular & …, 2018 - Springer
In the present study, Agrobacterium tumefaciens-mediated transformation of Glycine max
(L.) Merr.(soybean) cv. DS-9712 using half-seed explants was optimized for eight different …

Improved and reliable plant regeneration and Agrobacterium-mediated genetic transformation in soybean (Glycine max L.)

R Tiwari, AK Singh, MV Rajam - Journal of Crop Science and …, 2023 - Springer
An efficient plant regeneration and transformation system is a prerequisite for soybean crop
improvement. To establish such a regeneration system for soybean genotype JS 335, the …

Low phytate soybean: next generation metabolic engineering using CRISPR-Cas 9 genome editing technology

V Krishnan, M Jolly, A Sachdev - Journal of Plant Biochemistry and …, 2023 - Springer
Next-generation metabolic engineering widens the possibility of using plants as biofactories
for the mass production of metabolites. CRISPR/Cas9 system is the newest and the most …

RNAi-mediated resistance to yellow mosaic viruses in soybean targeting coat protein gene

A Kumari, A Hada, K Subramanyam, J Theboral… - Acta Physiologiae …, 2018 - Springer
In this genomic era, soybean has entrenched genomic database which offer an extensive
scope for improvement through genetic manipulation, although demand for transgenics …

'AGRODATE': a rapid Agrobacterium-mediated transient expression tool for gene function analysis in leaf discs

V Krishnan, J Jose, M Jolly, T Vinutha, R Kumar… - Journal of Plant …, 2020 - Springer
Transient gene expression utilizing syringe mediated agro infiltration offers a simple yet
efficient technique for various transgenic applications. Although soybean is commonly used …

Molecular Genetics of Biotic Stress Management for Crop Improvement

A Hada, MSM Jaabir, S Velmurugan… - … Management: Volume 1, 2022 - Springer
Biotic stress is one of the major factors caused by living organisms like bacteria, fungi,
viruses, insects, nematodes, arachnids and other pests and pathogens that represent a …