The lysis of cells in order to extract the nucleic acids or proteins inside it is a crucial unit
operation in biomolecular analysis. This paper presents a critical evaluation of the various …

Electroporation (EP) is a commonly used strategy to increase cell permeability for
intracellular cargo delivery or irreversible cell membrane disruption using electric fields. In …

Despite longstanding appreciation of gene expression heterogeneity in isogenic bacterial
populations, affordable and scalable technologies for studying single bacterial cells have …

Droplet microfluidics has rapidly emerged as one of the key technologies opening up new
experimental possibilities in microbiology. The ability to generate, manipulate and monitor …

Understanding molecular, cellular, genetic and functional heterogeneity of tumors at the
single-cell level has become a major challenge for cancer research. The microfluidic …

Exploring the sequence space of enzyme catalysts is ultimately a numbers game. Ultrahigh-
throughput screening methods for rapid analysis of millions of variants are therefore …

Cell lysis is a process in which the outer cell membrane is broken to release intracellular
constituents in a way that important information about the DNA or RNA of an organism can …

Therapeutic enzymes are valuable biopharmaceuticals in various biomedical applications.
They have been successfully applied for fibrinolysis, cancer treatment, enzyme replacement …

Single cell multimodal analysis is at the frontier of single cell research: it defines the roles
and functions of distinct cell types through simultaneous analysis to provide unprecedented …

Highlights•Many new methods for performing single-microbe transcriptomics have been
developed.•A new generation of microfluidics is streamlining microbial culture, manipulation …