Conformational heterogeneity and probability distributions from single-particle cryo-electron microscopy
Single-particle cryo-electron microscopy (cryo-EM) is a technique that takes projection
images of biomolecules frozen at cryogenic temperatures. A major advantage of this …
images of biomolecules frozen at cryogenic temperatures. A major advantage of this …
Cryo-EM: beyond the microscope
LA Earl, V Falconieri, JLS Milne… - Current opinion in …, 2017 - Elsevier
Highlights•The number of structures determined by cryo-EM is continuing to grow
rapidly.•Computational methods are helping resolve conformational heterogeneity.• …
rapidly.•Computational methods are helping resolve conformational heterogeneity.• …
Deep learning-based mixed-dimensional Gaussian mixture model for characterizing variability in cryo-EM
Structural flexibility and/or dynamic interactions with other molecules is a critical aspect of
protein function. Cryogenic electron microscopy (cryo-EM) provides direct visualization of …
protein function. Cryogenic electron microscopy (cryo-EM) provides direct visualization of …
Model-based local density sharpening of cryo-EM maps
Atomic models based on high-resolution density maps are the ultimate result of the cryo-EM
structure determination process. Here, we introduce a general procedure for local …
structure determination process. Here, we introduce a general procedure for local …
MDSPACE: Extracting continuous conformational landscapes from Cryo-EM single particle datasets using 3D-to-2D flexible fitting based on molecular dynamics …
This article presents an original approach for extracting atomic-resolution landscapes of
continuous conformational variability of biomolecular complexes from cryo electron …
continuous conformational variability of biomolecular complexes from cryo electron …
NMMD: Efficient cryo-EM flexible fitting based on simultaneous Normal Mode and Molecular Dynamics atomic displacements
Atomic models of cryo electron microscopy (cryo-EM) maps of biomolecular conformations
are often obtained by flexible fitting of the maps with available atomic structures of other …
are often obtained by flexible fitting of the maps with available atomic structures of other …
MDTOMO method for continuous conformational variability analysis in cryo electron subtomograms based on molecular dynamics simulations
Cryo electron tomography (cryo-ET) allows observing macromolecular complexes in their
native environment. The common routine of subtomogram averaging (STA) allows obtaining …
native environment. The common routine of subtomogram averaging (STA) allows obtaining …
DeepHEMNMA: ResNet-based hybrid analysis of continuous conformational heterogeneity in cryo-EM single particle images
I Hamitouche, S Jonic - Frontiers in Molecular Biosciences, 2022 - frontiersin.org
Single-particle cryo-electron microscopy (cryo-EM) is a technique for biomolecular structure
reconstruction from vitrified samples containing many copies of a biomolecular complex …
reconstruction from vitrified samples containing many copies of a biomolecular complex …
Deep generative modeling for volume reconstruction in cryo-electron microscopy
Advances in cryo-electron microscopy (cryo-EM) for high-resolution imaging of biomolecules
in solution have provided new challenges and opportunities for algorithm development for …
in solution have provided new challenges and opportunities for algorithm development for …
Computational methods for single-particle electron cryomicroscopy
A Singer, FJ Sigworth - Annual review of biomedical data …, 2020 - annualreviews.org
Single-particle electron cryomicroscopy (cryo-EM) is an increasingly popular technique for
elucidating the three-dimensional (3D) structure of proteins and other biologically significant …
elucidating the three-dimensional (3D) structure of proteins and other biologically significant …