Interest in CRISPR technology, an instrumental component of prokaryotic adaptive immunity
which enables prokaryotes to detect any foreign DNA and then destroy it, has gained …

Abstract Escherichia coli O157: H7, a Shiga-producing E. coli is a major pathogenic E. coli
strain which since the early 1980s has become a crucial food and water-borne pathogen …

Cell-free protein synthesis (CFPS) system is a simple, rapid, and sensitive tool that is devoid
of membrane-bound barriers, yet contains all the mandatory substrates, biomolecules, and …

Ideal methods for detecting pathogens should be sensitive, specific, rapid, cost-effective and
instrument-free. Conventional nucleic acid pathogen detection strategies, mostly PCR …

A main challenge in the development of biosensing devices for the identification and
quantification of nucleic acids is to avoid the amplification of the genetic material from the …

Biomedical researchers have subsequently been inspired the development of new
approaches for precisely changing an organism's genomic DNA in order to investigate …

An ideal molecular diagnostic method should be sensitive, specific, low cost, rapid, portable,
and easy to operate. Traditional nucleic acid detection methods based mainly on PCR …

A single gene mutation can cause a number of human diseases that affect the quality of life.
Until the development of clustered regularly interspaced short palindromic repeats …

The dengue virus (DENV) is a vector-borne flavivirus that infects around 390 million
individuals each year with 2.5 billion being in danger. Having access to testing is paramount …

CRISPR‐Cas systems are a form of prokaryotic adaptive immunity that employs RNA‐
guided endonucleases (Cas effectors) to cleave foreign genetic elements. Due to their …