Observation of molecular processes inside living cells is fundamental to a quantitative
understanding of how biological systems function. Specifically, decoding the complex …

Nucleic acids are excellent at recognizing complementary sequences through the formation
of Watson− Crick base pairs. Since the base pairing enables the highly selective …

Modern microscopic techniques following the stochastic motion of labelled tracer particles
have uncovered significant deviations from the laws of Brownian motion in a variety of …

Anyone who has used a light microscope has wished that its resolution could be a little
better. Now, after centuries of gradual improvements, fluorescence microscopy has made a …

A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a
fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic …

Abstract CHARMM (Chemistry at HARvard Molecular Mechanics) is a highly versatile and
widely used molecular simulation program. It has been developed over the last three …

Imaging single fluorescent proteins in living mammalian cells is challenged by out-of-focus
fluorescence excitation. To reduce out-of-focus fluorescence we developed reflected light …

Recently, graphene oxide (GO) based nanocomposites have raised significant interests in
many different areas, one of which being antibacterial agents where sliver nanoparticle …

Fluorescence provides a mechanism for achieving contrast in biological imaging that
enables investigations of molecular structure, dynamics, and function at high spatial and …

We have developed a single-molecule imaging technique that uses quantum-dot-labeled
peptide-major histocompatibility complex (pMHC) ligands to study CD4+ T cell functional …