FRET as a biomolecular research tool—understanding its potential while avoiding pitfalls
The applications of Förster resonance energy transfer (FRET) grow with each year.
However, different FRET techniques are not applied consistently, nor are results uniformly …
However, different FRET techniques are not applied consistently, nor are results uniformly …
Genetically encoded fluorescent biosensors illuminate the spatiotemporal regulation of signaling networks
EC Greenwald, S Mehta, J Zhang - Chemical reviews, 2018 - ACS Publications
Cellular signaling networks are the foundation which determines the fate and function of
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …
Patterning of the cell cortex by Rho GTPases
Abstract The Rho GTPases—RHOA, RAC1 and CDC42—are small GTP binding proteins
that regulate basic biological processes such as cell locomotion, cell division and …
that regulate basic biological processes such as cell locomotion, cell division and …
Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals spatially organized RAC1 signalling from integrin adhesions
PM Müller, J Rademacher, RD Bagshaw… - Nature cell …, 2020 - nature.com
Rho GTPases are central regulators of the cytoskeleton and, in humans, are controlled by
145 multidomain guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating …
145 multidomain guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating …
Rationally engineered nucleic acid architectures for biosensing applications
Development of biosensing platforms plays a key role in research settings for identification
of biomarkers and in clinical applications for diagnostics. Biosensors based on nucleic acids …
of biomarkers and in clinical applications for diagnostics. Biosensors based on nucleic acids …
High-sensitivity measurements of multiple kinase activities in live single cells
Increasing evidence has shown that population dynamics are qualitatively different from
single-cell behaviors. Reporters to probe dynamic, single-cell behaviors are desirable yet …
single-cell behaviors. Reporters to probe dynamic, single-cell behaviors are desirable yet …
Defined extracellular matrix compositions support stiffness-insensitive cell spreading and adhesion signaling
Integrin-dependent adhesion to the extracellular matrix (ECM) mediates mechanosensing
and signaling in response to altered microenvironmental conditions. In order to provide …
and signaling in response to altered microenvironmental conditions. In order to provide …
Frequency modulation of ERK activation dynamics rewires cell fate
Transient versus sustained ERK MAP kinase (MAPK) activation dynamics induce
proliferation versus differentiation in response to epidermal (EGF) or nerve (NGF) growth …
proliferation versus differentiation in response to epidermal (EGF) or nerve (NGF) growth …
Genetically encoded fluorescent indicators for imaging intracellular potassium ion concentration
Potassium ion (K+) homeostasis and dynamics play critical roles in biological activities. Here
we describe three genetically encoded K+ indicators. KIRIN1 (potassium (K) i on r atiometric …
we describe three genetically encoded K+ indicators. KIRIN1 (potassium (K) i on r atiometric …
Measuring signaling and RNA-seq in the same cell links gene expression to dynamic patterns of NF-κB activation
Signaling proteins display remarkable cell-to-cell heterogeneity in their dynamic responses
to stimuli, but the consequences of this heterogeneity remain largely unknown. For instance …
to stimuli, but the consequences of this heterogeneity remain largely unknown. For instance …