Whole genome amplification: abundant supplies of DNA from precious samples or clinical specimens

RS Lasken, M Egholm - TRENDS in Biotechnology, 2003 - cell.com
Whole genome amplification methods are used to generate the large amounts of DNA that
are required for genetic testing. Preparation of genomic DNA from clinical samples is a …

Degenerate oligonucleotide-primed PCR: general amplification of target DNA by a single degenerate primer

NP Carter, CE Bebb, M Nordenskjo, BAJ Ponder… - Genomics, 1992 - Elsevier
A version of the polymerase chain reaction (PCR), termed degenerate oligonucleotide-
primed PCR (DOP-PCR), which employs oligonucleotides of partially degenerate sequence …

Whole genome amplification from a single cell: implications for genetic analysis.

L Zhang, X Cui, K Schmitt, R Hubert… - Proceedings of the …, 1992 - National Acad Sciences
We have developed an in vitro method for amplifying a large fraction of the DNA sequences
present in a single haploid cell by repeated primer extensions using a mixture of 15-base …

Morphology and structure of polytene chromosomes

IF Zhimulëv - Advances in genetics, 1996 - Elsevier
Publisher Summary This chapter reviews the morphology and structure of polytene
chromosomes. The chapter focuses on the gene mapping and building of cytogenetic maps …

In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA

J Pinter, T Kurihara, I Sleptsova, E Bruening… - US Patent App. 10 …, 2004 - Google Patents
The present invention regards a variety of methods and compositions for whole genome
amplification. In a particular aspect of the present invention, there is a method of amplifying a …

Whole-genome multiple displacement amplification from single cells

C Spits, C Le Caignec, M De Rycke, L Van Haute… - Nature protocols, 2006 - nature.com
Multiple displacement amplification (MDA) is a recently described method of whole-genome
amplification (WGA) that has proven efficient in the amplification of small amounts of DNA …

Whole genome amplification using a degenerate oligonucleotide primer allows hundreds of genotypes to be performed on less than one nanogram of genomic DNA

VG Cheung, SF Nelson - Proceedings of the National …, 1996 - National Acad Sciences
Genetic analysis of limiting quantities of genomic DNA play an important role in DNA
forensics, paleoarcheology, genetic disease diagnosis, genetic linkage analysis, and …

Whole genome analysis of genetic alterations in small DNA samples using hyperbranched strand displacement amplification and array–CGH

JM Lage, JH Leamon, T Pejovic, S Hamann… - Genome …, 2003 - genome.cshlp.org
Structural genetic alterations in cancer often involve gene loss or gene amplification. With
the advent of microarray approaches for the analysis of the genome, as exemplified by array …

Mapping Polycomb-repressed domains in the bithorax complex using in vivo formaldehyde cross-linked chromatin

V Orlando, R Paro - Cell, 1993 - cell.com
Summary The Polycomb group (PC-G) proteins are responsible for keeping developmental
regulators, like homeotic genes, stably and inheritably repressed during Drosophila …

Optimization and evaluation of single‐cell whole‐genome multiple displacement amplification

C Spits, C Le Caignec, M De Rycke… - Human …, 2006 - Wiley Online Library
The scarcity of genomic DNA can be a limiting factor in some fields of genetic research. One
of the methods developed to overcome this difficulty is whole genome amplification (WGA) …