Gel-filtration chromatography

C Ó'Fágáin, PM Cummins, BF O'Connor - … chromatography: Methods and …, 2011 - Springer
Gel-filtration chromatography is a popular and versatile technique that permits the effective
separation of proteins and other biological molecules in high yield. Here, the basis of the …

Gel-filtration chromatography

C Ó'Fágáin, PM Cummins, BF O'Connor - … Chromatography: Methods and …, 2017 - Springer
Gel-filtration chromatography is a versatile method that permits the effective separation of
biological molecules in high yield. This article describes the basis of the method, the …

Protein and virus-like particle adsorption on perfusion chromatography media

Y Wu, J Simons, S Hooson, D Abraham… - … of Chromatography A, 2013 - Elsevier
The structural and protein adsorption characteristics of the perfusion chromatography matrix
POROS® HS 50 are determined. Transmission electron microscopy shows a broad …

Chromatographic techniques: types, principles, and applications

VBC Kumari, SM Patil, R Ramu, PS Shirahatti… - Analytical techniques in …, 2022 - Elsevier
Chromatography is regarded as one of the most significant bioanalytical techniques used in
different branches of life sciences and chemistry. It allows the separation, identification, and …

Comparison of perfusion media and monoliths for protein and virus-like particle chromatography

Y Wu, D Abraham, G Carta - Journal of Chromatography A, 2016 - Elsevier
Structural and performance characteristics of perfusion chromatography media (POROS HS
20 and 50) and those of a polymethacrylate monolith (CIM SO3-1 tube monolith column) are …

Immunization with a DNA vaccine candidate in chronic hepatitis C patients is safe, well tolerated and does not impair immune response induction after anti‐hepatitis B …

M Castellanos, Z Cinza, Z Dorta, G Veliz… - The journal of gene …, 2010 - Wiley Online Library
Background In the present study, we evaluated the safety of CIGB‐230, a novel vaccine
candidate based on the mixture of a plasmid for DNA immunization, expressing hepatitis C …

Aminoglycoside antibiotic-derived anion-exchange microbeads for plasmid DNA binding and in situ DNA capture

TSP Grandhi, A Mallik, KN Lin, B Miryala… - … applied materials & …, 2014 - ACS Publications
Plasmid DNA (pDNA) therapeutics are being investigated for gene therapy and DNA
vaccines against diseases including cancer, cystic fibrosis and AIDS. In addition, several …

[图书][B] Scalable technology to produce pharmaceutical grade plasmid DNA for gene therapy

OR Hernández, ML Fernández, J Valdes… - 2011 - books.google.com
The use of gene therapy is a promising process for the prevention, treatment and cure of
diseases such as cancer and acquired inmudeficiency syndrome (AIDS); increasing a …

Simulation for the recovery of plasmid for a DNA vaccine

M Limonta, NL Krajnc, U Vidic… - Biochemical engineering …, 2013 - Elsevier
The pIDKE2 plasmid is the main component of the CIGB's candidate vaccine against
Hepatitis C virus (HVC), which is being used in HCV chronically-infected individuals during …

Toxicology and biodistribution study of CIGB-230, a DNA vaccine against hepatitis C virus

D Bacardí, Y Amador-Cañizares… - Human & …, 2009 - journals.sagepub.com
CIGB-230, a mixture of a DNA plasmid expressing hepatitis C virus (HCV) structural
antigens and a HCV recombinant capsid protein, has demonstrated to elicit strong immune …