Deconstructing Cell-Free Extract Preparation for in Vitro Activation of Transcriptional Genetic Circuitry

AD Silverman, N Kelley-Loughnane… - ACS synthetic …, 2018 - ACS Publications
Recent advances in cell-free gene expression (CFE) systems have enabled their use for a
host of synthetic biology applications, particularly for rapid prototyping of genetic circuits and …

A highly productive, one-pot cell-free protein synthesis platform based on genomically recoded Escherichia coli

BJ Des Soye, VR Gerbasi, PM Thomas, NL Kelleher… - Cell chemical …, 2019 - cell.com
The site-specific incorporation of non-canonical amino acids (ncAAs) into proteins via amber
suppression provides access to novel protein properties, structures, and functions …

The protection role of magnesium ions on coupled transcription and translation in lyophilized cell-free system

X Guo, Y Zhu, L Bai, D Yang - ACS Synthetic Biology, 2020 - ACS Publications
Cell-free protein synthesis (CFPS) is a promising platform for protein engineering and
synthetic biology. The storage of a CFPS system usually involves lyophilization, during …

Synthetic biology at the hand of cell-free systems

A Pandi, O Borkowski, JL Faulon - Advances in Synthetic Biology, 2020 - Springer
Cell-free systems are emerging as membraneless transcriptional and translational tools to
study biology and for prototyping, characterization, and engineering novel biological …

Cell-free protein synthesis platforms derived from clostridia extracts

MC Jewett, A Kruger-Gericke, AP Mueller… - US Patent App. 16 …, 2020 - Google Patents
Disclosed are compositions, methods, and kits for performing cell-free RNA transcription
and/or cell-free protein synthesis (CFPS). The disclosed compositions, methods, and kits …

Cell-free protein synthesis platforms derived from clostridia

MC Jewett, A KRUGER-GERICKE, AP MUELLER… - 2020 - Google Patents
WO2020176522A1 - Cell-free protein synthesis platforms derived from clostridia - Google Patents
WO2020176522A1 - Cell-free protein synthesis platforms derived from clostridia - Google Patents …