For decades, budding yeast, a single-cellular eukaryote, has provided remarkable insights
into human biology. Yeast and humans share several thousand genes despite …

At present, the great challenge in human genetics is to provide significance to the growing
amount of human disease-associated gene variants identified by next generation DNA …

Precise Hox gene expression is crucial for embryonic patterning. Intra-Hox transcription
factor binding and distal enhancer elements have emerged as the major regulatory modules …

Although transplantation of single genes in yeast plays a key role in elucidating gene
functionality in metazoans, technical challenges hamper humanization of full pathways and …

The capability to manipulate and analyze hard-wired metabolic pathways sets the pace at
which we can engineer cellular metabolism. Here, we present a framework to extensively …

The construction of microbial cell factories for sustainable production of chemicals and
pharmaceuticals requires extensive genome engineering. Using Saccharomyces cerevisiae …

The widely used Streptococcus pyogenes Cas9 (SpCas9) nuclease derives its DNA
targeting specificity from protein-DNA contacts with protospacer adjacent motif (PAM) …

Algae are diverse organisms with significant biotechnological potential for resource
circularity. Taking inspiration from fermentative microbes, engineering algal genomes holds …

Yeast research is entering into a new period of scholarship, with new scientific tools, new
questions to ask and new issues to consider. The politics of emerging and critical technology …

Given the broad utility of humanized yeast to model and study human biology, a reference
set of human genes that can replace cognate yeast genes and operate in yeast is needed …