Cholesterol anchors enable efficient binding and intracellular uptake of DNA nanostructures

WL Whitehouse, JE Noble, MG Ryadnov… - Bioconjugate …, 2019 - ACS Publications
DNA nanostructures constitute a rapidly advancing tool-set for exploring cell-membrane
functions and intracellular sensing or advancing delivery of biomolecular cargo into cells …

Binary encoding of random peptide sequences for selective and differential antimicrobial mechanisms

Z Hayouka, A Bella, T Stern, S Ray… - Angewandte Chemie …, 2017 - Wiley Online Library
Binary encoding of peptide sequences into differential antimicrobial mechanisms is
reported. Such sequences are random in composition, but controllable in chain length, are …

A Nonlinear Peptide Topology for Synthetic Virions

JE Noble, YW Hsiao, IE Kepiro, E De Santis, A Hoose… - ACS …, 2024 - ACS Publications
a nonlinear de novo peptide topology for the assembly of synthetic virions is reported. The
topology is a backbone cyclized amino-acid sequence in which polar l-and hydrophobic d …

Folding-mediated DNA delivery by α-helical amphipathic peptides

JE Noble, P Vila-Gómez, S Rey, C Dondi… - ACS Biomaterials …, 2023 - ACS Publications
The renaissance gene therapy experiences these days requires specialist biomaterials and
a systemic understanding of major factors influencing their ability to deliver genetic material …

Designer protein pseudo-capsids targeting intracellular bacteria

S Rey, N Faruqui, A Hoose, C Dondi… - Biomaterials …, 2021 - pubs.rsc.org
The emergence of multidrug-resistant bacteria stimulates the search for antimicrobial
materials capable of addressing challenges conventional antibiotics fail to address. The …

Peptide nanoparticles for gene packaging and intracellular delivery

P Vila-Gómez, JE Noble, MG Ryadnov - Polypeptide Materials: Methods …, 2021 - Springer
Efficient gene transfer is necessary for advanced biotechnologies ranging from gene therapy
to synthetic biology. Peptide nanoparticles provide suitable packaging systems promoting …

Revealing sources of variation for reproducible imaging of protein assemblies by electron microscopy

IE Kepiro, B Nardone, A Page, MG Ryadnov - Micromachines, 2020 - mdpi.com
Electron microscopy plays an important role in the analysis of functional nano-to-
microstructures. Substrates and staining procedures present common sources of variation …

Self-assembly at the multi-scale level: challenges and new avenues for inspired synthetic biology modelling

G Milano, I Marzuoli, CD Lorenz, F Fraternali - 2017 - books.rsc.org
We describe here progresses in the field of molecular simulations of large, macromolecular
assemblies of different nature, ranging from protein complexes to protein–lipids, virus-like …

Designer proteins for bottom-up synthetic biology

MG Ryadnov - 2017 - books.rsc.org
This chapter discusses recent developments in sub-and extra-cellular biological systems
assembled from designer polypeptides. Artificial and semi-designed extracellular matrices …