[HTML][HTML] Pseudomonas putida as a functional chassis for industrial biocatalysis: from native biochemistry to trans-metabolism

PI Nikel, V de Lorenzo - Metabolic engineering, 2018 - Elsevier
The itinerary followed by Pseudomonas putida from being a soil-dweller and plant colonizer
bacterium to become a flexible and engineer-able platform for metabolic engineering stems …

The ArcAB two-component system: function in metabolism, redox control, and infection

AN Brown, MT Anderson, MA Bachman… - Microbiology and …, 2022 - Am Soc Microbiol
ArcAB, also known as the Arc system, is a member of the two-component system family of
bacterial transcriptional regulators and is composed of sensor kinase ArcB and response …

From dirt to industrial applications: Pseudomonas putida as a synthetic biology chassis for hosting harsh biochemical reactions

PI Nikel, M Chavarria, A Danchin… - Current opinion in chemical …, 2016 - Elsevier
Highlights•SynBio needs chassis with superior genetic, biochemical, and physiological
traits.•Environmental bacteria are endowed with many advantageous metabolic properties.• …

Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression

E Martínez-García, PI Nikel, T Aparicio… - Microbial cell …, 2014 - Springer
Background Because of its adaptability to sites polluted with toxic chemicals, the model soil
bacterium Pseudomonas putida is naturally endowed with a number of metabolic and stress …

Genome reduction boosts heterologous gene expression in Pseudomonas putida

S Lieder, PI Nikel, V de Lorenzo, R Takors - Microbial cell factories, 2015 - Springer
Background The implementation of novel platform organisms to be used as microbial cell
factories in industrial applications is currently the subject of intense research. Ongoing …

New transposon tools tailored for metabolic engineering of Gram-negative microbial cell factories

E Martínez-García, T Aparicio, V de Lorenzo… - … in bioengineering and …, 2014 - frontiersin.org
Re-programming microorganisms to modify their existing functions and/or to bestow bacteria
with entirely new-to-Nature tasks have largely relied so far on specialized molecular biology …

Metabolic and regulatory rearrangements underlying glycerol metabolism in Pseudomonas putida KT2440

PI Nikel, J Kim, V De Lorenzo - Environmental microbiology, 2014 - Wiley Online Library
While the natural niches of the soil bacterium P seudomonas putida are unlikely to include
significant amounts of free glycerol as a growth substrate, this bacterium is genetically …

The glycerol-dependent metabolic persistence of Pseudomonas putida KT2440 reflects the regulatory logic of the GlpR repressor

PI Nikel, FJ Romero-Campero, JA Zeidman… - MBio, 2015 - Am Soc Microbiol
The growth of the soil bacterium Pseudomonas putida KT2440 on glycerol as the sole
carbon source is characterized by a prolonged lag phase, not observed with other carbon …

[图书][B] Microbial cell factories engineering for production of biomolecules

V Singh - 2021 - books.google.com
Microbial Cell Factories Engineering for Production of Biomolecules presents a compilation
of chapters written by eminent scientists worldwide. Sections cover major tools and …

Refactoring the Embden–Meyerhof–Parnas pathway as a whole of portable GlucoBricks for implantation of glycolytic modules in Gram-negative bacteria

A Sánchez-Pascuala, V de Lorenzo… - ACS synthetic …, 2017 - ACS Publications
The Embden–Meyerhof–Parnas (EMP) pathway is generally considered to be the
biochemical standard for glucose catabolism. Alas, its native genomic organization and the …