Plant metabolism as studied by NMR spectroscopy

C Deborde, A Moing, L Roch, D Jacob, D Rolin… - Progress in nuclear …, 2017 - Elsevier
The study of plant metabolism impacts a broad range of domains such as plant cultural
practices, plant breeding, human or animal nutrition, phytochemistry and green …

Metabolic flux analysis in plants: coping with complexity

DK Allen, IGL Libourel… - Plant, cell & environment, 2009 - Wiley Online Library
Theory and experience in metabolic engineering both show that metabolism operates at the
network level. In plants, this complexity is compounded by a high degree of …

Metabolomic analysis of key central carbon metabolism carboxylic acids as their 3‐nitrophenylhydrazones by UPLC/ESI‐MS

J Han, S Gagnon, T Eckle, CH Borchers - Electrophoresis, 2013 - Wiley Online Library
Multiple hydroxy‐, keto‐, di‐, and tri‐carboxylic acids are among the cellular metabolites of
central carbon metabolism (CCM). Sensitive and reliable analysis of these carboxylates is …

Low-temperature effect on enzyme activities involved in sucrose–starch partitioning in salt-stressed and salt-acclimated cotyledons of quinoa (Chenopodium quinoa …

M Rosa, M Hilal, JA Gonzalez, FE Prado - Plant physiology and …, 2009 - Elsevier
The effect of low temperature on growth, sucrose–starch partitioning and related enzymes in
salt-stressed and salt-acclimated cotyledons of quinoa (Chenopodium quinoa Willd.) was …

Integrated 13C-metabolic flux analysis of 14 parallel labeling experiments in Escherichia coli

SB Crown, CP Long, MR Antoniewicz - Metabolic engineering, 2015 - Elsevier
The use of parallel labeling experiments for 13 C metabolic flux analysis (13 C-MFA) has
emerged in recent years as the new gold standard in fluxomics. The methodology has been …

Tracking the metabolic pulse of plant lipid production with isotopic labeling and flux analyses: past, present and future

DK Allen, PD Bates, H Tjellström - Progress in lipid research, 2015 - Elsevier
Metabolism is comprised of networks of chemical transformations, organized into integrated
biochemical pathways that are the basis of cellular operation, and function to sustain life …

Carbon and nitrogen provisions alter the metabolic flux in developing soybean embryos

DK Allen, JD Young - Plant Physiology, 2013 - academic.oup.com
Soybean (Glycine max) seeds store significant amounts of their biomass as protein, levels of
which reflect the carbon and nitrogen received by the developing embryo. The relationship …

Understanding fatty acid synthesis in developing maize embryos using metabolic flux analysis

AP Alonso, VL Dale, Y Shachar-Hill - Metabolic Engineering, 2010 - Elsevier
The efficiency with which developing maize embryos convert substrates into seed storage
reserves was determined to be 57–71%, by incubating developing maize embryos with …

Metabolic network fluxes in heterotrophic Arabidopsis cells: stability of the flux distribution under different oxygenation conditions

TCR Williams, L Miguet, SK Masakapalli… - Plant …, 2008 - academic.oup.com
Steady-state labeling experiments with [1-13C] Glc were used to measure multiple metabolic
fluxes through the pathways of central metabolism in a heterotrophic cell suspension culture …

Central metabolic fluxes in the endosperm of developing maize seeds and their implications for metabolic engineering

AP Alonso, DL Val, Y Shachar-Hill - Metabolic Engineering, 2011 - Elsevier
14C labeling experiments performed with kernel cultures showed that developing maize
endosperm is more efficient than other non-photosynthetic tissues such as sunflower and …