Clusters of bacterial RNA polymerase are biomolecular condensates that assemble through liquid–liquid phase separation
AM Ladouceur, BS Parmar… - Proceedings of the …, 2020 - National Acad Sciences
Once described as mere “bags of enzymes,” bacterial cells are in fact highly organized, with
many macromolecules exhibiting nonuniform localization patterns. Yet the physical and …
many macromolecules exhibiting nonuniform localization patterns. Yet the physical and …
Emerging Quantitative Biochemical, Structural, and Biophysical Methods for Studying Ribosome and Protein–RNA Complex Assembly
Ribosome assembly is one of the most fundamental processes of gene expression and has
served as a playground for investigating the molecular mechanisms of how protein–RNA …
served as a playground for investigating the molecular mechanisms of how protein–RNA …
[HTML][HTML] Escherichia coli NusG links the lead ribosome with the transcription elongation complex
It has been known for more than 50 years that transcription and translation are physically
coupled in bacteria, but whether or not this coupling may be mediated by the two-domain …
coupled in bacteria, but whether or not this coupling may be mediated by the two-domain …
Single-molecule tracking reveals the functional allocation, in vivo interactions, and spatial organization of universal transcription factor NusG
H El Sayyed, OJ Pambos, M Stracy, ME Gottesman… - Molecular Cell, 2024 - cell.com
During transcription elongation, NusG aids RNA polymerase by inhibiting pausing,
promoting anti-termination on rRNA operons, coupling transcription with translation on …
promoting anti-termination on rRNA operons, coupling transcription with translation on …
Transcription complexes as RNA chaperones
N Said, MC Wahl - Transcription, 2021 - Taylor & Francis
To exert their functions, RNAs adopt diverse structures, ranging from simple secondary to
complex tertiary and quaternary folds. In vivo, RNA folding starts with RNA transcription, and …
complex tertiary and quaternary folds. In vivo, RNA folding starts with RNA transcription, and …
Structure-based mechanisms of a molecular RNA polymerase/chaperone machine required for ribosome biosynthesis
Bacterial ribosomal RNAs are synthesized by a dedicated, conserved transcription-
elongation complex that transcribes at high rates, shields RNA polymerase from premature …
elongation complex that transcribes at high rates, shields RNA polymerase from premature …
Major-groove sequence-specific RNA recognition by LoaP, a paralog of transcription elongation factor NusG
A Elghondakly, MD Jermain, WC Winkler… - Structure, 2024 - cell.com
LoaP is a member of the universal NusG protein family. Previously, we reported that unlike
other characterized homologs, LoaP binds RNA sequence-specifically, recognizing a stem …
other characterized homologs, LoaP binds RNA sequence-specifically, recognizing a stem …
Roles of the leader-trailer helix and antitermination complex in biogenesis of the 30S ribosomal subunit
Ribosome biogenesis occurs co-transcriptionally and entails rRNA folding, ribosomal
protein binding, rRNA processing, and rRNA modification. In most bacteria, the 16S, 23S …
protein binding, rRNA processing, and rRNA modification. In most bacteria, the 16S, 23S …
Nusbiarylins Inhibit Transcription and Target Virulence Factors in Bacterial Pathogen Staphylococcus aureus
The emergence of multidrug resistance in the clinically significant pathogen Staphylococcus
aureus is a global health burden, compounded by a diminishing drug development pipeline …
aureus is a global health burden, compounded by a diminishing drug development pipeline …
Transcription termination and antitermination of bacterial CRISPR arrays
A hallmark of CRISPR-Cas immunity systems is the CRISPR array, a genomic locus
consisting of short, repeated sequences ('repeats') interspersed with short, variable …
consisting of short, repeated sequences ('repeats') interspersed with short, variable …