After three decades of developments, single particle tracking (SPT) has become a powerful
tool to interrogate dynamics in a range of materials including live cells and novel catalytic …

Optical microscopy has for centuries been a key tool to study living cells with minimum
invasiveness. The advent of single molecule techniques over the past two decades has …

Tau amyloid assemblies propagate aggregation from the outside to the inside of a cell,
which may mediate progression of the tauopathies. The critical size of Tau assemblies, or" …

Single-plane illumination (SPIM) or total internal reflection fluorescence (TIRF) microscopes
can be combined with fast and single-molecule-sensitive cameras to allow spatially resolved …

Multicolor imaging based on genetically encoded fluorescent proteins (FPs) is a powerful
approach to study several dynamic processes in a live cell. We report a monomeric orange …

The RelA-mediated stringent response is at the heart of bacterial adaptation to starvation
and stress, playing a major role in the bacterial cell cycle and virulence. RelA integrates …

Biomolecular condensates serve as membrane-less compartments within cells,
concentrating proteins and nucleic acids to facilitate precise spatial and temporal …

Biochar (BC) is a carbon-rich material that can be obtained by thermal decomposition of
agricultural solid waste under oxygen-limited conditions. It has received increasing attention …

Optical detection of single molecules mostly relies on their fluorescence because of the high
contrast of this technique against the background. Since their invention in the early 1990s …

In the recent decade, single-molecule (sm) spectroscopy has come of age and is providing
important insight into how biological molecules function. So far our view of protein function is …